Effects Of Captopril And Xin Fu Kang Koufuye On GLUT4 Of Myocardial In Experimental Congestive Heart Failure Rats

Objective: The paper focuses on the effect of captopril and traditional Chinese medicine Xin Fu Kang Koufuye on function of invigoration QI warming YANG and promoting blood circulation to removing blood stasis on Glucose Transporter Protein 4 of myocardium in pressure overload-induced left ventricular hypertrophy in rats. Also it discusses the change of expression for GLUT4 of the glucose metabolism in process of CHF as well as the mechanism for ameliorate of the glucose metabolism in CHF.Methods: The method of coarctation of abdominal aorta (CAA) in male SD rats was taken to make high pressure overload-induced left ventricular hypertrophy model of CHF. 80 SD rats were randomly divided into four groups: Sham operation(SH), Coarctation of abdominal aorta model group (CAA), Captopril Tablets cure group(CAP) and Xin Fu Kang Koufuye group(XFK). And then measure the function of heart and grayscale of GLUT4 protein by Western Blot after six and ten weeks respectively and observe the change of them.Result: 1.The function of Heart: (1) In the time of 6 week: compare with SH group, HR, SAP, DAP, LVEDP and LVMI of CAA group increased(P<0.01), +dp/dtmax and -dp/dtmax decreased(P<0.01). Compare with SH group , HR, DAP, LVEDP of CAP and XFK group increased(P<0.01), +dp/dtmax and -dp/dtmax decreased(P<0.01). Compare with CAA group, HR, LVMI, DAP and LVEDP of CAP and XFK group decreased (P<0.01), +dp/dtmax and -dp/dtmax increased (P<0.01). Betwe- en CAP and XFK group, all the stat value is same (P>0.05) except +dp/dtmax(P<0.05). (2) In the time of 10 week: compare with SH group, SAP, DAP, HR, LVEDP and LVMI of CAA group increased, +dp/dtmax and -dp/dtmax decreased (P<0.01). Compare with SH group, LVEDP of CAP and XFK group decreased(P<0.01), +dp/dtmax and -dp/dtmax increased (P<0.01). Compare with CAA group, SAP, LVMI, HR, DAP and LVEDP of CAP and XFK group decreased(P<0.01), +dp/dtmax and -dp/dtmax increased(P<0.01). Between CAP and XFK group, all the stat value is same(P>0.05). (3) Compare with 6th, LVMI and LVEDP of CAA group in the 10th increased (P<0.05), +dp/dtmax and -dp/dtmax decreased (P<0.05). LVMI and LVEDP of CAP and XFK group in the 10th decreased (P<0.05), and +dp/dtmax increased(P<0.05) with -dp/dtmax not change.2.the Grayscale(GLUT4/GAPDH)of GLUT4 protein in the 6th week: (1) The study shows that CAA group GLUT4 protein ma- rkedly decreased compare with SH group, CAP group and XFK group (P<0.01). (2) In the CAP group the grayscale of GLUT4 protein markedly decreased compare with SH group (P<0.01) while indiscriminate compare with XFK group (P>0.05). (3) In the XFK group the grayscale of GLUT4 protein markedly decreased compare with SH group(P<0.01).3.the Grayscale(GLUT4/GAPDH) of GLUT4 protein in the 10th week: (1)The study shows that the grayscale of GLUT4 protein of CAA group markedly decreased compare with SH group, CAP group and XFK group (P<0.01). (2) In the CAP group the grayscale of GLUT4 protein markedly decreased compare with SH group(P<0.01) while indiscriminate compare with XFK gro- up(P>0.05). (3) In the XFK group the grayscale of GLUT4 pro- tein is indiscriminate compare with SH group(P>0.05).Conclusion: (1)The expression of glucose transporter protein 4 is decreased with the harmfulness of heart function by high pressure overload-induced CHF. (2) Captopril can enhance and improve the heart function and the expression of GLUT4 of myocardial of Rat by high pressure overload-induced CHF. (3) Xin Fu Kang Koufuye on function of invigoration QI warming YANG and promoting blood circulation to removing blood stasis can enhance and improve the heart function and the expression of GLUT4 of myocardial of Rat by high pressure overload-induced CHF.