Effect Of Bionics-pulsed Electromagnetic Field On The Femur Head Necrosis

Objective : To study the effect of bionics—pulsed electromagnetic field(BEMF) on the femur head necrosis.Means : In this test we adopt horse serum to inject creating the rabbit hypersensitivity blood vessel inflammation , allying with the adrenal cortex hormone to lead to the femur head necrosis , which means we inject the sterilized horse serum through the ear venous of our matured rabbits , 10ml/kg/time for twice , and the intermission is 3 weeks ; Then Injects the Methylpoednisolone (MP) celiac 40mg/ kg/ day for 3 days , After the last time inject the femur head necrosis mode should be established . The instrument which gives the pulse electromagnetic fields treatment is provided by China medical institute, and such fundamental parameter is as follows : The wave pattern is the pointed top, attenuated wave pattern; The frequency in pulsed magnetic fields is 2 - 20Hz ;The largest magnetic field intensity is 10mT; The whole range of the signal power is 0-250 Hz, and mainly focus in 2-75 Hz. The experimental animal is 40 from which we randomly collect 10 as the blank comparison group(C), not offering any treating . Remaining 30 are randomly divided into tow groups (A, B ),each contains 15. The A group builds the femur head necrosis mode and meanwhile offers the eletromagnetic fields to each rabbit for preventive treatment, 1 hour /day. The treating comparison of B group only build the standard mode. All groups are killed 8 weeks after the last injection of MP, acting blood checkup, pathology, and immunohistochemistry.Select the patients of femoral head necrosis and all the cases are without collapse(pre the III b by ARCO).We treated 16 casers and the mean age is 54ys(44-67ys),the meant time of suffering is 10 ys(3-20ys).The post-steroid cases is 6, post-truma is 8 cases and the idiopathic is 2 cases. All of the patients were treated by the BEMF 1 hour/day. And we examined effect of the BEMF by the Harris Hip Rating Scale and ARCO classification of the cases pre and post the treatment.Result : 1 .blood checkup reveals : In C group the concentration of fibrin in plasma are obviously lower than the other groups which show no difference .The alkaline phosphatase show no difference in all groups; 2. the pathology checkup displays : In treating comparison group the trabecula become thinner and sparser with disorganized frame, even disconnection. The nuclear of the bone cells shrinks, deeply dyed. The cells in the marrow which can form blood reduce, while fatty cells increase. Empty lacunae is up to 32.2 %. In A group, there are much improvement(21.7% ). But they are differ from normal which can be seen in the blank comparison group (16.3%). The amount of vascular in group C is larger than other groups. 3.The immunohistochemistry suggested the up-regulated expression of PPAR gamma in A and B group. And the PPAR gamma expression of A group was lower than B group.In clinical treatment we revealed the BEMF can release the pain of the hip. By the treatment of BEMF 13 of the cases have obvious development in the Harris scale and none have a aggravation. The ARCO have no change in all cases.Conclusion : 1. The high concentration of fibrin in plasma caused by adrenal cortex hormone may be one of the cause of femur head necrosis. 2. The PPAR gamma decrease at the early period of the AVN. 3. Pulse electromagnetic fields may reduce the PPAR gamma of the AVN rabbits. And it can decreased the adipocyte and increased ossteoblast. It can do some help to prevent femur head necrosis from developing and may be one of the working therapy .4. The clinical effect of the BEMF mainly is the released the pain of hip.