Inhibiting SDF-1 Impact Immunoregulatory Function Of MSCs Supernatant And Proliferation Of Leukemia Cell Line HL-60

ObjectiveTo investigate the immunoregulation of MSCs supernatant on proliferation of allogeneic T lymphocytes activated by PHA and Proliferation of Acute Myelocytic Leukemia Cell line HL-60 when the effects of SDF-1 were inhibited.MethodsBone marrow mesenchymal stem cells were isolated by Ficoll-Hypaque density gradient, and the surface marks were detected by flow cytometry. Allogeneic T lymphocytes from peripheral blood were harvested by nylon column and HL-60 cells were conventional cultured. SDF-1 activity was blocked with anti-CXCR4 McAb 12G5. Supernatant of MSCs was co-cultured with T lymphocytes activated by PHA and HL-60 cells. Proliferation of cells were detected by MTT. The secretion of the cytokines IL-4 and INF-γwere evaluated by ELISA.ResultsAfter SDF-1 activity was blocked with anti-CXCR4 McAb 12G5, the enhancement of MSCs supernatant on HL-60 cells was inhibited and the inhibition of MSCs supernatant on T lymphocytes was not obviously effected. The secretion of IL-4 were 8.27±2.68pg/ml,22.84±5.61pg/ml,5.35±2.51pg/ml and the secretion of INF-γwere 11.75±4.95 pg/ml,165.81±14.73 pg/ml,186.99±45.09pg/ml in the T-cells alone group, T-cells and MSCs supernatant co-culture group and adding McAb 12G5 group respectively.Conclusion Blocking SDF-1 activity can inhibit proliferation of leukemia cells and has no obvious effect on proliferation of allogeneic T Lymphocytes inhibited by MSCs supernatant and secretion of IFN-γ. The secretion of IL-4 was decreased.