Expression Of NF-κB P50 And P16^INK4A In The Lesions Of Condyloma Acuminatum And Cervical Cancer

The study can be divided into two parts.The First Part Objective:To investigate the expression of NF-κB p50 and P16 INK4A in condyloma acuminatum(CA)with HPV6/11 positive.Methods:21 cases of HPV 6/11 positive and HPV16/18 negative were diagnosed by Fluorescent quantitative PCR(FQ-PCR).The expression of NF-κB p50 and P16^INK4Awere detected by immunohistochemistry technique with 21 cases of CA lesions and 18 cases of normal human skins(foreskin).Results: NF-κB p50 expression mainly localizes in the cytoplasm,few can be found to express in nucleus,The expression of NF-κBp50 in normal foreskin and 21 case of CA appears a significantly difference,(Z=-4.412,P＜0.05).P16^INK4Apositive expression mainly localizes in the cytoplasm and the expression of P16^INK4Ain the two groups also show a significantly difference(Z=-2.669,P＜0.05).There is no evidence to conform the relationship of the expression of NF-κB p50 and P16^INK4Ain the lesions of CA.(r_s=0.009,P＞0.05),Conclusions:NF-κB p50 and P16^INK4Amay be involved in the cell proliferation and control in the lesions of CA with HPV6/11 positive. The Second Part Objective:Expression of NF-κBp50 and P16^INK4Ain CA,CIN and cervical cancer.three tissues with HPV16/18 positive and HPV6/11 negative were diagnosed by in situ hybridization.Methods:Immunohistochemistry(LDP)to detect the expression of P16^INK4Aand NF-κBp50 in 21 cases CA,24 cases CINI,10 cases CIN11 and 15 cases cervical cancer patients respectively.Resuls:The positive rate of P16^INK4Aprotein in CA was 14.28%,The positive rate in CIN and cervical cancer are both 100%.The expression of P16^INK4Aprotein in CA,CINI,CIN11 and the cervical cancer had a significantly difference(p＜0.05).The positive rate of NF-κBp50 protein in CA,CINI,CIN11 and cervical cancer were 100%respectively,a The expression of NF-κBp50 protein in the group of CA,CINI,CIN11 and the cervical cancer show significantly difference(P＜0.05).Conclusions:Activated NF -κBp50 may play an role in human papillomavirus infection tissue.The positive expression of P16^INK4Aappears in CIN and cervical cancer,which means it can be take as a biomarker for CIN screening and diagnosis of ealier cancer.