Effects Of Erythropoietin On Proliferation And Cell Circle Stage Of Human Bone Marrow Mesenchymal Stem Cells In Vitro

ObjectiveTo study the effects of different doses of erythropoietin (EPO) on the proliferation and the cell cycle's of human mesenchymal stem cells (MSCs) .MethodsMesenchymal stem cells were isolated from bone marrow by the methods of density centrifugation;Percoll's solution was used, under serum free culture media, MSCs were incubated with different doses of EPO( 0.25, 0.50, 1.00, 2.00, 4.00,8.00U/mL),and then the proliferation of MSCs was measured by MTT assays after 24h,48h,72h ;Cell circle stage was measured by flow cytometry assays after incubation of 10 U/ml EPO for 72 h.ResultsAfter MSCs was incubated with EPO,the cell proliferation index of MSCs was significant.EPO enhance MSCs' proliferation in a dose and time dependent manner.The effects was highest in 4U/mL group. Compared with control group EPO could significantly decrease G0/G1 ratio, and increase S and G₂/M stage ratio.ConclusionUnder the EPO culture condition can promote more mesenchymal stem cells enter the stage of DNA synthesis.It is, therefore,more and more cells can divide and produce a lot of proliferation cells , this effect is in a dose and time depenedent manner.