| It was reported that cancer was in the first place of the death causes in Shunde, Guangdong and primary liver cancer (PLC) mortality was the first in Cancer from the Third Time of Nationwide Death Cause Sample Survey in 2006.PLC mortality was 49.84/1,000,000 above national average in Shunde in 2005.PYLL of PLC occupied cancer 38.60%.removed PLC,the resident can live 1.21 years longer.The people was severity threaten by PLC. But the studies on epidemiology of Primary Live Cancer in Shunde were seldom reported. The risk factors of PLC were not identify .So, it was necessary explore risk factors of PLC in Shunde City and this work had a certain realistic significance.Purposes:To performed a case-control study to explore risk factors of PLC, to research the association between genetic polymorphisms of the human tumor necrosis factor-α-308 position and susceptibility to PLC, and to probe the interaction between environmental and genetic factors in hepatocarcinogenesis by applying molecular biological technology .To analyse the birth orders of PLC cases, trying to know the relative roles of environmental and genetic factors in Hepatocarcinogenesis.Objects and methods:Subjects were PLC patient in hospital from 2004 to 2007 and sibling who lived in Shunde above 10 years. The controls who didn't have PLC, not blood relation with patient at the same village, same sex, age±3 years, living in Shunde above 10 years.The contents of survey include:(1) Questionnaire: general conditions;personal history of smoking, drinking,eating, drink water,disease status, birth order, etc;(2)Laboratory examination:○1 to detect HBV, HCV,AFP, liver fluke by ELISA .○2 to analyze TNF-α-308 position by PCR-RELP.We first applied conditional logistic regression analysis to explore the main effects and interactional effects of HBV, smoking, drinking,TNF2in hepatocarcinogenesis. Then we analyzed the birth orders of PLC cases.Results:(1) The frequencies of allele TNF2 were significant differences between case group and control group (P=0.012), with the OR of 2.67 (95%CI: 1.24-5.74).(2) Applied conditional logistic regression to explore risk factors of PLC , Hepatitis B, alcohol consumption, liver desease history were associated with increased risks of PLC in this study.(3) HBsAg was interacted with the TNF2 allele during the generation of PLC, With the OR=19.83(95%CI:4.20-93.61); their RERI were 14.39, their API were 0.7253,their SI were 4.23.(4) Liver desease history was interacted with the TNF2 allele during the generation of PLC, With the OR=7.63(95 %CI:1.05-67.71),their RERI were 2.22, their API were 0.29,their SI were 1.50.(5) Alcohol consumption was interacted with the TNF2 allele during the generation of PLC, With the OR=4.57(95 %CI:1.62-12.88),their RERI were 1.39, their API were 0.30,their SI were 1.63. (6) No relation was found between birth order and pathogenesis of PLC in the patient of HBsAg (-),but in the patient of HBsAg(+) birth order had relation with pathogenesis of PLC.Conclusion:(1) There was association between genetic polymorphisms of the human tumor necrosis factor-α-308 position and PLC; the TNF2 allele was susceptibility to PLC.(2) Applied conditional logistic regression to explore risk factors of PLC , Hepatitis B, alcohol consumption, liver desease history were associated with increased risks of PLC in this study.(3) The TNF2 allele was interacted with HBsAg, liver desease history, alc-ohol consumption.(4) Hepatitis B played more important role than genetic factors in hepatica-rcinogenes in Shunde.But control Hepatitis B, genetic factors would play important role in hepatocarcinogenesis .
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