The Significance And The Expression Of Desmin And Dystrophin In The Musculus Obliquus Internus Abdominis Of The Old Patients With Inguinal Hernia.

【Objectives】.Present work aimed to observe the relativity of the expression of cytoskeleton protein desmin in cytoplasm and dystropin in cytolemma of the musculus obliquus internus abdominis of the old male patients with inguinal hernia.to detect the microstructure in the musculus obliquus internus abdominis of the old patients with inguinal hernia,and to explore whether the alteration of expression of desmin and dystrophin could contribute to the old patients with inguinal hernia.【Methods】.Forty old patients with inguinal hernia as research group,twenty age-matched appendicitis patients without inguinal hernia as control group,biopsy samples were obtained from patients from the same location of the musculus obliquus internus abdominis.The change of desmin and dystrophin in muscle cell was studied by immunohistochemical and electron microscopic techniques.【Results】.1.The image explains.,muscle fibre in the structure of muscle tissue in control group are tight interrelate,there are collagen textile fiber,fibroblast,vein and nerve in muscle bundle.Among them,muscle fibre is homogeneously dyeinged pink, cell nucleus locate in the border of muscle fibre,no muscle fibre shows degeneration and necrosis.The density of muscle fibre in research group is reduced,arrange comparatively sparse.A small amount muscle fibre which be filled and replaced by comparatively rich fibre connective tissue show island shape scatter,and be infiltrated by Inflammatory cell.Muscle fibre shrink obviously,is uneven dyeinged.there are many center of cell nucleus are shifted,few musle fibre have vacuolation effect.HE explains:P＜0.05.The image of muscle tissue signigicantly change in research group,compared to the control group.2.Immunohistochemical and electron microscopic techniques explains:The expression of desmin significantly reduced in research group,compared to the control group(P＜0.05).The expression of dystrophin significantly reduced in research group,compared to the control group(P＜0.01).【Conclusions】.In this study,the evidences which we found provide morphologic basis suggesting that the significantly reduced expression of desmin and dystrophin maybe contribute to the pathogenesis of old patients with inguinal hernia.