The Expression Of TRAF6 In Experimental Periodontitis With Rats

Objective (1) To construct an animal sample for the experimental periodontitis with rats, and through observing the clinical manifestation and pathological changes in each phase of the rats periodontitis, provide experimental conditions for further study of related pathogenic factors and function control in the proceeding and developing process of periodontitis. (2) To observe the expression of TRAF6 in each phase of rat periodontitis pathological changes, and discuss its relation with periodontal disease in its occurrence and development.Methods (1) Construct an experimental model of rat periodontitis: randomly divide 40 rats into four groups, namely, the normal comparable group, 4\0 thread-stitched group, No.1 black non-absorbable surgical suture group, and orthodontics group; 10 rats per group. Stitch the upper first molars' cervix in both sides of the rats respectively with 4\0 thread, No. 1 black nonabsorbent surgery suture and orthodontics ligation wire. Then kill the rats 2w, 4w and 6w after the ligation respectively. Intercept upper jaw bone including experimental teeth and periodontal organization., and then make the histology specimens with HE staining, in order to observe the changes of the rats' periodontal organization in different period and the features of organizing pathology.(2) Observe TRAF6's expression features of experimental periodontitis at different stages with immunohistochemical methods based on experimental periodontitis in the rat model, and then analyze them in statistic way.Results (1) Construct an experimental model of rat's periodontitis with ligation methods. The results show the following:the gum of experimentation teeth with the 2w group of ligation shows a little red with blood when probing examined, the histology observation shows that: the epidermis and connective tissue of the gum of experimentation teeth has a great deal of heterophil granulocyte being soakaged, the periodontal film arrange in order, the frame of alveolar bone is integrated, which accords with the symptom of the forepart gum inflammation. 4w group of ligation shows that the surface of the experimentation teeth has a few dental calculi attached, the dental gum swell and appear blood when probing examined, and 1.0 mm of shallow periodontal pocket form when probing examined. The histology observation shows that: the wall of gum channel has a great deal of inflammation cell being soakaged, the epidermis and connective tissue of gum channel appear glue fibril denaturalization and proliferate to the side of root with the epidermis, and the height of the tooth groove lowered, which accord with the symptom of the forepart gum inflammation. The 6w group of ligation shows that homologous chap mucous membrane swells with blood apparently when probing examined, 1.2 mm of shallow periodontal pocket has formed, the teeth groove bone absorb in evidence, and the teeth root exposes. The histology observation shows that: the rats' gum corrupts and putresces, proliferates and extends to the side of root with the epidermis, then forms the deep periodontal pocket, who is surrounded by dense inflammation cells. The teeth groove absorbs them and was destroyed, so formed obvious broken bone cells and bone absorbing lacuna, which accords with the symptom of the later period of the gum inflammation. Comparable group clinical observation shows that: the rats' teeth gum appear to be pink, its texture is tough, and doesn't bleed when probing examined; the histology observation shows that: the rats' periodontal film is arranged in order, the surface of the alveolar bone is lubricous, and the broken bone cells and bone lacuna haven't formed. The results above indicate that the construction of experimental model of rat's periodontitis is successful and able to offer base for the next experiment. (2)The expression of TRAF6: immunohistochemical results showed that in the normal comparable group, TRAF6 has negative expression in gum and periodontal ligament but weakly positive expression in alveolar bone. In the 2w group, TRAF6 has positive expression in the gum organization, negative expression in the periodontal ligament, and weekly positive expression in the alveolar bone. In the 4w group, TRAF6 has positive expression in gum and alveolar bone and strongly positive expression in periodontal ligament. In the 6w group, TRAF6 has strongly positive expression in the whole periodontal organization, which exists in the edge of alveolar bone whose bone absorption has been destroyed greatly, but not shows out in the alveolar bone cells. The analysis result through integral ray density about the experimental results shows that: As the disease became more and more serious, the TRAF6's expression increased apparently. Group 2w, 4w and 6w have enhanced their positive area by 1.06 times, 1.62 times and 2.78 times compared to the normal group. 4w group has enhanced its positive area 0.27 times compared to the2wgroup. 6w group has enhanced its positive area 0.25times and 0.59 times compared to group 2w and 4w respectively. The difference between all the experimental group after the surgery has its statistically significance. (P < 0.05) .Conclusion: (1)Setting up the rat model with ligation wire can stimulate the natural process of periodontitis' development, and systematically observe the rat periodontitis progress, in order to provide the experimental basis for further research on the gene which will cause periodontal diseases. (2)With the development of the disease, the expression of TRAF6 goes upwards in the periodontal lesions. It shows that TRAF6 is involved in the inflammatory cell signal transduction, and plays an important role in the biological aspect. The result provides a theoretical basis and experimental basis for the further exploration of TRAF6's regulatingfunction in periodontitis inflammation cells.