Experimental Study Of The Optic Neuroprotective Effect Of Cannabinoid(WIN55, 212-2) By Topical Application It

Objective: To evaluate the optic neuroprotective effect of the cannabinoid(WIN55,212-2) by topical application(eyedrop) it to chronic glaucoma rabbit model.Methods: 16 rabbits randomly divided into 4 groups. Made all the rabbits into chronic glaucoma model beside the normal control group(8 eyes). 7 days later, the high IOP control group(8 eyes) eyedrop application with normal saline, the timolol group(8 eyes) and cannabinoid group(8 eyes) eyedrop application with 0.5%timolol and 0.125%WIN55-212-2. The number of the retinal ganglion cells(RGCs) and the glumatic acid density of vitreous body are observed and quantified in the specimens.Results: when we topical application timolol to chronic glaucoma rabbit for 4 weeks, the low IOP effect is not significant, and the IOP isn't normal. But when we use WIN55,212-2, the IOP is lower evidently, and approximate normal. By comparison the number of RGCs and the glumatic acid density of vitreous body, there was statistical significant different between cannabinoid group and high IOP control group/timolol group(p<0.05), while cannabinoid group showed more RGCs and lower glumatic acid density of vitreous body. There was almost no difference between cannabinoid group and normal control group(p>0.05).Conclusion: WIN55,212-2 topical application could produce the optic neuroprotective effect on the chronic glaucoma rabbit.