| Objective:As a common and frequently-occurring disease with high disability and mortality, the disease of diabetes has been regarded as a serious threat to human health and a heavy burden to society and family. The inflammatory immune responses together with the diabetic complications are associated with the occurrence of cerebral infarction, which plays an important role in diabetes. C-reactive protein (CRP) has been emerged as a sensitive biomarker for inflammatory process. The increase of CRP is believed to be related to the poor prognosis of cerebral infarction. However, the exact clinical importance of CRP still needs to be clarified. Amyloid beta-peptide (Aβ) is the major component of senile plaque in the brains of Alzheimer's disease (AD) patients. The Aβmainly composed by Aβ40 and Aβ42 is the proteolytic product of the larger beta amyloid precursor protein (βAPP) and the initial factor for neuronal injuries. Neurons are damaged by the Aβvia the excitotoxic pathway including the accumulation of extracellular glutamate, the activation of N-methyl-D-aspartate (NMDA) receptor and the overloading of intracellular Ca2+ ion et al. Aβalso has tight connection with the inflammatory response in the brain of AD patient. The levels of AβandβAPP change in response to the damages of diabetic endothelial cell. However, the level change and the biological implications of Aβduring the process of endothelial cell injuries are unknown. And the relationships among the various pathogenic factors remain unclear.In order to reveal the change correlation between the Aβlevel and the CRP in the patients suffering from diabetes, we measured the levels of Aβ40, Aβ42 and CRP in the bloods of 0-day-admission and 7-day-admission diabetic patients, respectively. The changes of Aβ40, Aβ42 and CRP in patients'bloods were investigated and their clinical implications were discussed.Methods:Twenty-four patients suffering from diabetes (0-day-admission and 7-day-admission in hospital) and sixteen healthy persons were selected and subjected to the measurements of CRP and Aβ40 in serum and Aβ42 in plasma. Aβ40 in serum and Aβ42 in plasma were measured by double sandwich enzyme linked immunosorbent assay (ELISA).Results:1. Comparing to the control group, the level of Aβ40 increase in the serum of diabetic group patients (P<0.01), while the level of Aβ42 decrease in the plasma of diabetic group patients (P<0.01), which shows a negative correlation. The levels of Aβ42 in the plasma of diabetic patients (0-day-admission and 7-day-admission) were found positively correlated with the ages of patients (P<0.05), which indicate that the older patients have higher level of Aβ42 than younger patients (r=0.424, P<0.01; r=0.309, P<0.05).2. The levels of CRP in the serum of diabetic group patients were observed increase with significance comparing to those of the control group persons (P<0.01).3. There is a positively correlation between the Aβ40 and CRP in the serum of diabetic patients (P<0.05).Conclusions:1. The level of Aβ40 in the serum of diabetic patients has a negative correlation with the level of Aβ42 in the plasma of diabetic patients. Plasma Aβ42 levels are increased in older patients.2. The Aβ40 level is positively correlated with CRP.
|
PDF Full Text Request