Research On Neuroprotective Function Of Recombined Human Erythropoietin In Rat Spinal Cord Injury

Objective To study the neuroprotective function of recombined human erythropoietin in rat spinal cord injury model, especially from the suppression of swelling of spinal cord tissue, lipid peroxidation and apoptosis aspect, the possible mechanism of neuroprotection was approached, to find out a new method of therapy to treat the spinal cord injury.Method 65 SD rats were divided randomly into four groups including sham operation group, spinal cord injured group, rhEPO treated group and MP treated group. The modified Allen's model of spinal cord injury was established, the neurofunctional recovery was observed according to BBB score, MDA, SOD and TUNEL labeling, including behavior, lipid peroxidation, apoptosis and structural changes.Result Treated with rhEPO(1000U/kg), the neurofunction of rat is outweigh that of SCI group and MP treated group on the 7th day and 14th day, and recovery time also shorter than MP treated group. Compared with SCI group, rhEPO treated group had lesser TUNEL positive cells and more neuron remained without distinction to MP treated group, the levels of MDA of which were lower than those of SCI group, and the levels of SOD were higher in early stage. The levels of MDA and SOD in all groups were negative correlative(r=-0.785).Conclusion rhEPO can suppress the lipid peroxidation progress in early stage of injured spinal cord, reduce the apoptosis of neurons in spinal cord, so that neuroprotective function can be gained by the usage of rhEPO.