Detection Of FUS-CHOP Fusion Gene Transcript And The Expression Of MDM2 And P53 In Myxiod/round Cell Liposarcoma

objective To explore the expression of FUS-CHOP mRNA and MDM₂,P53 protein in Myxiod/round cell Liposarcoma and their significance.Methods:We detected FUS-CHOP fusion transcripts by Nested reverse transcription-polymerasase chain reaction using RNA extracted from archival formalin-fixed,paraffin-embedded tissue in twenty-five MRCL samples and 18 control cases,followed by DNA sequencing to confirm the PCR product.β-actin gene was used to assess the quality of the mRNA templates.All of the MRCL cases was Available for Immunohistochemical analysis of M-DM₂ and p53 status.Results:β-actin mRNA was detected in 18 MRCLs(18/25,72%)and 12 negative control case(12/18,66.7%).Typeâ…¡FUS-CHOP mRNA was successfully detected in 15 out of 25(60%)MRCLs and 15 out of 18(83.3%)MRCLs excluded of the cases that were negative forβ-actin mRNA. All of control cases were negative for the FUS-CHOP fusion gene transcripts.The positive rate of MDM2 and p53 proteins were 56%(14/25)and 44%(11/25),respectively,both of them were significantly higher in round cell liposarcoma than in myxiod and mixed liposarcoma,There was no correlation between FUS-CHOP mRNA and the expression of p53,MDM₂ protein,but there was a tendency of positive correlation between the expression of MDM₂ and p53.Conclusions:(1)FUS-CHOP is considered as specific molecular and genetic hallmark for MRCLs and is useful for the diagnosis of MRCLs.(2)The overexpressed of MDM₂ and p53 protein is associated with the progression of MRCLs.(3)There is a no correlation between MDM₂,P53 and FUS-CHOP in MRCLs.