Study On Chemical Constituents From The Rhizome Of Helleborus Thibetanus Franch. (Ⅱ)

Helleborus Thibetanus Franch., belonging to the family of Ranunculaceae, is a kind of chinese traditional medicines used in some regions of QinLing mountain, and its phytochemistry has not been studied. In this paper, the progress on chemical compositions and pharmacological research were reviewed.The systematic pretests have been carried out by the tube-test methods. The results showed that the Helleborus Thibetanus Franch. contained carbohydrates, flavonoids, steroid saponins, organic acids, volatile oils etc., no alkaloids, thraquinone and its glycosides.Nine compounds were isolated and purified from the n-butanol extracts of the rhizome of Helleborus Thibetanus in conjuction with the use of silica gel, silica gel 60, macroporous adsorption resins, Sephedex LH-20 column chromatography and re-crystallization. Based on the analysis of their spectral data (MS, UV, IR, 1D 1HNMR,13CNMR and 2D HSQC, HMBC, 1H-1H COSY, ROESY) and the comparation with the related literature data, the eight compounds were identified. Two novel compounds were identified as 14β,16β-dihydroxy-5β-bufa-20,22-dienolide-3β-O-β-D-glucoside (5) and 14β,16β-dihydroxy-5β-bufa-20,22-dienolide-3β-O-β-D-glucose(1→4)-O-β-D-glucoside (4). Other six known compounds were identified as 5β-hydroxyecdysterone (1),β- ecdysterone (2), 3,5,14-trihydroxy-19-oxo-3-[(α-L-rhamnopyranosyl)oxy]bufa-20,22- dienolide (3) , hellebrigenin (6), glucose (8), rutin(9).