Studies On Extraction And Purification Of Flavones From Fructus Aurantii Immaturus

Fructus Aurantii Immaturus are the immature fruits of Citrus aurantium L..It has the function of dispelling qi and eliminating the accumulation,reducing phlegm and dispersing the ruffian.It mainly contains volatile oil,alkaloids and flavones which have strong anti-inflammatory and antioxidant and antiallergic activity.We study on analysis of flavones in Fructus Aurantii Immaturus and extraction process of flavones in Fructus Aurantii Immaturus and purification process of flavones in Fructus Aurantii Immaturus with Macroporous resin. The results are following:1.Study on analysis of flavones in Fructus Aurantii ImmaturusThe experiment determined the analysed method of content of flavones in Fructus Aurantii Immaturus by the HPLC with naringin and neohesperidin as control.The results show that there exist significant differences in intrinsic quality among Fructus Aurantii Immaturus of different specifications.As the diameter of Fructus Aurantii Immature become bigger,the content of naringin is higher and the content of neohesperidin is lower.2.Study on extraction process of flavones in Fructus Aurantii ImmaturusApplied celhlase enzymolysis and pectinase enzymolysis in extraction of the flavones in Fructus Aurantii Immaturus.The results show that there don't exist significant differences adding enzymolysis or not.Confirmation of the best extractive technics of the flavone of Fructus Aurantii Immaturus through the four factors(concentration of ethanol, dosage of extractive solvent,time of extraction,times of extraction)three levels orthogonal test,the results show that the optimized process parameters are the extraction of Fructus Aurantii Immaturus with 6 times 70%ethanol for 3 times,1 hour per time.3.Study on purification process of flavones in Fructus Aurantii Immaturus with Macroporous resinThe absorption and desorption test are used to select the better resin.The results show that the adsorption effect are different with diffterent types acroporous resin and the LSA-40 resin are the suitable material.Based on the result above,with the content of naringin and neohesperidin as indexes,the kinesis adsorb and elution process with quantity of naringin and neohesperidin are investigated,the maximum quantity and density and pH of the physic liquor and the type and quantity of elution solvent are determined,the results show that maximum quantity of Fructus Aurantii Immaturus the each gramma wet resin can adsorb is 0.45g,and the elution solvent determined is three times column volume of 60%alcohol,and the content of naringin in the extract is 28.8%,neohesperidin in the extract is 48.0%.So the refine process can enrich the flavones in Fructus Aurantii Immaturus effectively.