| Traditional Chinese Patent Medicine plays an important role in Chinese medicine. It includes herbal medicine, traditional Chinese medicine injections and traditional Chinese medicine formula. Its characteristics are complexity in composition and "multiple target action". Since the quality of Traditional Chinese Medicine determines the efficiency which is important to both operation mechanism research and clinical effect, Chinese physicians always pay great attention to the estimation and quality control of Traditional Chinese Medicine.The quality estimation of Traditional Chinese Medicine is different from that of western medicines. If only the major components' content in Traditional Chinese Medicine are measured just done as in western medicines. It cannot reflect the quality of Traditional Chinese Medicine exactly and disobeys the theory of Traditional Chinese Medicine and the "multiple target effect". In the same way, it is too difficult to separate all the hundreds, even thousands of components and to analyze their contents quantitatively, which also collides with the estimation's desire to be exact, more simple and convenient.Traditional Chinese Medicine fingerprint spectra technique is one approbatory way to estimate quality of Traditional Chinese Medicine in recent years, and is popular with all the world now. Not limited in the quality estimation of western medicine, many kind imaginabale experiments are used to collect information on chemical ingredients in Traditional Chinese Medicine. With the help of mathematics and statistics the data are dealt with and right conclusions are achieved finally .The operation is simple and convenient, too. For the moment fingerprint spectra technique is mostly applied to the quality estimation of both herbal medicine, traditional Chinese medicine injections and extracts from herbal medicines. Nowadays, however, fingerprint spectra technique is not applyied to the quality estimation of formula frequently, that is because much more complex characteristics are in formula than that in herbal medicines and traditional Chinese medicine injections.My study aims at applying IR and HPLC fingerprint technique, which is two major means used in fingerprint technique, to collect relevant information from three similar Dihuang Pills (Guifu Dihuang Pill, Jinkui Dihuang Pill and Qiju Dihuang Pill), and dual-index sequence analysis (two indexes: common peak ratio and variant peak ratio) are carried out based on the measured data. The quality estimation of three similar Dihuang Pills (Guifu Dihuang Pill, Jinkui Dihuang Pill and Qiju Dihuang Pill) then are performed.In the study of IR fingerprint spectra: the IR spectra of both original powder (standing for the whole components) and three kinds of extracts with chloroform, ethanol and water (standing for three different part of polar composition, and all of them can nearly be considered as the whole components) of every Dihuang Pill were collected and processed. The estimating conclusions are made respectively, and the results were compared with each other. The effects of different degree of measuring sensitivity on the conclusions are also analyzed. In the study of HPLC fingerprint spectra, the HPLC fingerprint spectra of ethanolic extracts of every Dihuang Pill were determined and analyzed in detail. The results are compared with that of IR fingerprint spectra.The estimating conclusions of IR fingerprint spectra of Dihuang Pills show that the same kind of Dihuang Pill are highly similar, howover, different kinds of Dihuang Pill are distinct obviously. The estimating conclusions of IR fingerprint spectra of all the three extracts of Dihuang Pill can not only achieve the purpose to tell Dihuang Pills apart but also to identify and differentiate their manufacturers and batches. Comparing the estimating conclusion of original powder's IR fingerprint spectra with that of extracts, the latter can provide more detail information and its capability of judgment is even more useful. The estimating conclusions of HPLC fingerprint spectra of ethanolic extracts indicate that Dihuang Pills coming from the same company and batch are extraordinarily alike, especially, which accords with the truth.The results offered by our work show that the Dual index sequence analytical method combining with statistics is more effect on identifying and evaluating formula-the extremely complex Traditional Chinese medicines.
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