Study Of The Number And The Expression Of Focal Adhension Kinase In Circulation Endothelial Cells In Haemorrhagic Fever Of Renal Syndrome

Objective To study the expression the phosphorylation state of serine 910 of focal adhesion kinase(FAK ps910)in circulating endothelial cells(CEC)and the number of CEC in hemorrhagic fever of renal syndrome (HFRS)and analyze the relation between FAK ps910 expression and endothelial injury.Methods Fifty patients with HFRS were involved in the study including 17 mild patients,20 moderate patients and 13 severe patients according to the illness condition.And twenty healthy volunteers were designed as the control group.For each person,after the CEC was isolated by Isopyknic Centrifugation method,the expression of FAK ps910 was got from Flow Cytometry.And The CEC number was determined with Flow Cytometry too.Statistics method was used to analyze the difference between HFRS and normal subjects and among the three types and different stages of HFRS.Results In patients with different stage of HFRS,the number of CEC were statistically higher(except recovery phase)than those in the controls. The number of CEC was related with the clinical condition.During late fever stage and hypotension stage,the expression of FAK ps910 in mild,moderate,severe and control group each was 17.45±2.64%,13.84±2.54%,7.47±2.57%and 21.14±2.53%.There is an obvious relation between decrease of FAK ps910 and illness severity.FAK ps910 level increased obviously in fever stage(p＜0.05),decreased under the normal line in the late phase of fever stage and hypotension stage(p＜0.05) and then restored normal gradually.Conclusions The CEC provided a new way to study the pathogy of HFRS.The number of CEC changed regularly according to the clinicaltypes and the stages.FAK maybe involve in the signal transduction associated with intergrinβ3and plays an important role in endothelial injury related to Hantavirus virus.FAK ps910 can forcast the clinical severity in the early stage of HFRS.