Study On Mobilization Of Tanshinol And Ligustrazine On Peripheral Blood Hematopoietic Stem Cells In Mice

ObjectiveTo study tanshinol and ligustrazine for mobilizing peripheral blood hematopoietic stem cells in BABL/c mice and approach the possible mechanism of its mobilization in the cellular and molecular level .MethodsTwenty-four BABL/c mice were divided into four groups at random(n=6 each): From day 1 to 7,mice were intraperitoneally injected with tanshinol 300mg.kg-1.d-1 (Group Tanshinol),ligustrazine 50mg.kg-1.d-1(Group ligustrazine),and rhG-CSF 250 ug.kg-1.d-1(Group rhG-CSF) respectively,normal control with equivalence Saline.The changes of WBC and MNC at different time after treatment of the mice were observed. Another forty BABL/c mice were divided into four groups at random(n=10 each):From day 1 to 7,mice were intraperitoneally injected with tanshinol 300mg.kg-1.d-1 (Group Tanshinol),ligustrazine 50mg.kg-1.d-1(Group ligustrazine),and rhG-CSF 250 ug.kg-1.d-1(Group rhG-CSF) respectively,normal control with equivalence Saline. On the eighth day, the effects of the numbers of WBC and MNC were observed by means of WBC and MNC counts,the percentage of CD34+ cell and CD49d positive cell were observed by means of flow cytometry,the yields of CFU-GM,CFU-MK,CFU-E in peripheral were observed by means of hematopoietic cell culture in vitro and the percentage of VCAM-1 positive cells in bone marrow stromal cells of the mice were observed by means of immunocytochemistry.Results8 days after be treated of group tanshinol, the numbers of WBC and MNC reached the maximum, which were 3.0 times and 3.4 times of the NS group respectively; the percentage of CD34+ cells and CD49d positive cells in peripheral blood and VCAM-1 positive cells in bone marrow stromal cells in group tanshinol was (1.03±0.24)%,(12.59±2.64)%and(50.86±8.77)% respectively ,were markedly higher than in NS group(P<0.05); the yields of CFU-GM,CFU-MK and CFU-E in group of tanshinol was (14.90±2.88)%,(12.50±4.06)% and(16.10 ±6.36)% respectively, were markedly higher than in NS group(P<0.01); 8 days after be treated of group ligustrazine, the numbers of WBC and MNC reached the maximum, which were 3.2 times and 3.9 times of the NS group respectively; the percentage of CD34+ cells and CD49d positive in peripheral blood and VCAM-1 positive cells in bone marrow stromal cells in group ligustrazine was (0.86±0.42)%,(12.91±2.84)% and (48.47±7.87)% respectively ,were markedly higher than in NS group (P<0.05); the yields of CFU-GM,CFU-MK and CFU-E in group of tanshinol was (11.70±3.32)%,(11.20±2.88)% and (24.50±8.10)% respectively, were markedly higher than in NS group(P<0.01).ConclusionThe results indicate that tanshinol and ligustrazine could mobilize hematopoietic stem cells into peripheral blood .And this role may be related to the increase adhesion molecule expression in peripheral blood cells and mouse bone marrow stromal cell.