Effect On Acupuncture Of Stimulating Du Channel And Smoothing Other Channels To NF-kB In Synovium Of AA

Objective:To explore the expressions of Nuclear factor kB in the synovialis of the adjuvant arthritis of rats(AA),discuss the mechanism of the AA from adjust gene.in this way we expect can provide a more efficient,more security method for RA's treatment.Methods:Project four rat groups of bland control,model control, routine,acupuncture(RA)and Acupuncture of Stimulating Du Channel and Smoothing Other Channels and Collaterals(ASDCSOCC)group,study on the AA:(1)Observe the changes of the arthron swelling,ache grade of in flect arthron,synoviales.(2)Detecting the expressions of Nuclear factor kV(P65)in the synovilis and by using the immunity organization chemistry (IOC)and pathology change.Results:(1)Comparing with the blank control group,the level of the articulations swelling,flex articulations ache and arthrophlogosis of the model control group,and they had distinct disparity(P＜0.05,P＜0.01).After treating by the RA and acupuncture of Collaterals,all of the norm above had reduced,and they had distinct disparity comparing with the model control group(p＜0.05,p＜0.01).The ASDCSOCC group and the RA had distinct disparity(p＜0.05).(2)The immunity organization chemistry staining of the synoviales observation,the pathology integration of the model control group is raised clearly comparing with the blank control group and thy had distinct disparity(p＜0.01).After trreating,the pathology integration of the RA and ASDCSOCC are descending,comparing with the model control group they had distinct disparity(p＜0.01).Comparing with the RA group,the pathology intergration of the ASDCSOCC group is lower,and they had distinct disparity(p＜0.05).(3)The immunity organization chemistry staining of the synoviales NF-kB(P65)display the masculine reactant of the NF-kB(P65)emboldening clearly,and form like the ball distribution,and the color of the nucleolus in the staining is deeper than in the cytoplasm.The model control group is higher than the bland control group,and they had distinct disparity(P＜0.01).After treating by the RA and ASDCSOCC,comparing with the model control group thy had distinct disparity(P＜0.05,P＜0.01).Comparing with the ASDCSOCC group and the RA group,they had distinct disparity(P＜0.05).Conclsion:(1)The level of the metatarsus swelling,arthrophlogosis and flex articulations ache of AA qre reduced after treating by the RA and ASDCSOCC,and have the very clearly effect of antephlogistic, apocatastasis and analgecize,and the method of Acupuncture of Stimulating Du Channel and Smoothing Other Channels and Collaterals better than the routine acupuncture.(2)The expressions of Nuclear factor kB in the synovialil of the adjuvant arthritis of rats(AA)are raising,and the NF-kB joined in the etiopathogenesis of the whole body pathologic changed,they form the networt of the nerve-immunity.(3)The spine nerve pathology injury of the Aaare reduced by treating of the method of the ASDCSOCC and the synoviales is a one of the most important factors of the etiopathogenesis of RA,and the effect of the ASDCSOCC method is better than the RA.This is the obe od the unbalance label of the synoviales by the acupuncture treating. (4)It has very clearly adjustment effect of the AA by the acupuncture,to cure the RA and reach to natural state of the Yin and Yang Equilibrium by the traditional Chinese medicine mechanism.