Effect Of Acupuncture Preconditioning On C-fos Protein In Myocardium Of Rat With Myocardial Ischemia Reperfusion Injury

Objective: To investigate effect of acupuncture preconditioning on c-fos protein in myocardium of rat with myocardial ischemia reperfusion injury, to evaluate the effect of acupuncture PreconditioningMethods: 30rats were divided into five groups randomly in the experiment: normal group,sham-operation group,ischemic group,ischemic preconditioning group, acupuncture preconditioning group. This thesis adopts needle prick vessel model rats before myocardial ischemia. Through using "pushing pipe"-tie off the rat coronary artery left anterior descending branch and establish the model of rat myocardial ischemia reperfusion. HE stains to observe change of histomorphology. Immunohistochemistry test c-fos protein expression.Results: Acupuncture at "Nei Guan","Tan zhong " can reduce expression of c-fos protein in myocardium of rats with myocardial ischemia reperfusion injury. Acupuncture preconditioning group and ischemic reperfusion model group (IR)was significantly differenct (P <0.05), acupuncture preconditioning group compared with ischemic preconditioning (IP) was not significantly different (P> 0.05).Conclusion: Acupuncture preconditioning can lower c-fos protein expression,induce ischemic tolerance,reduce myocardial ischemia reperfusion injury,played the role of myocardial protection.