Research On The Expression Of The Vascular Endothelial Growth Factor Following Acute Cerebral Ischemia Reperfusion In Rats By The Electric Acupuncture On Nuchal Region

This project is to proceed with how the electric acupuncture on nuchal region (EANR) intervene the vascular endothelial growth factor ( VEGF). To investigate the mechanism of this aetion will pioneer a new realm of science research.Objective : To study the expression of VEGF following cerebral ischemia reperfusion (CIR) in rats and the influence of EANR. Try to reveal the mechanism of the therapy for CIR.Methods: Focal cerebral ischemia was induced by intraluminal middle cerebral artery occlusion ( MCAO) method using a monofilament thread in rats. Male Wistar rats were divided randomly in to four groups: sham group, model group, electric acupuncture on four limbs (EAFL) group and EANR group; each group was separated into 12h, 1d. 3d, 5d after reperfusion as subgroup for investigation.(1)Neurological deficits were observed in rats; (2) HE staining was used to research the changes of histomorphology; ( 3) The expression of VEGF were measured by immunohisto chemistry technique.Results: (1) Neurological deficit scores in EAFL and EANR group therapy groups were significantly higher than the ones in the model group (P<0.01, P<0.05) . And there was significant difference between the EANR and EAFL group (P<0.05). ( 2)The results of HE staining showed that after acupuncture there is light neural cell swelling but more neurons and newborn blood capillaries compared with the model group. ( 3) Immunohist ochemistry results indicated that compared with the model group, EAFL and EANR could obviously increase the expression of VEGF (P<0.01), and there was significant difference between the EANR and EAFL group (P<0.05) .Conclusions : ( 1 ) EAFL and EANR can save the neurocytes in peri-infarct region and reinforce the blood supply so as to amend neural function and reduce the infarction ratio.( 2)EAFL and EANR can increase the expression of VEGF so as to promote rehabilitation of neuronal function after focal ischemia and alleviate the injury induced by CIR. (3) Through comparison we find that the EANR acts better than the ANR in the aspects hereinbefore which related to the effect of the EANR which can improve the blood supply of the cerebrum.