Study On The Distribution Of Class Ⅰ Integron In Escherechia Coil Clinical Isolates And Its Correlation With Drug Resistance

ObjectUnderstand the characteristics of the clinical distribution for E. coli in North Sichuan Medical College Affiliated Hospital, analyze its drug resistance status, detect the positive rates of the class 1 integron and ESBLs in E. coli, and illuminate the mechanism of horizontal transmission of the resistance gene in order to provide the basic data for the prevention of drug resistance transmission and the clinically rational use of drugs.Methods1. Specimens from the clinical divisions were collected. The pathogens from specimens were cultivated, isolated, as well as identified and classified with VITEK 32 automatic system.2. The resistance of E. coli to 15 antibacterial drugs was detected by Micro-dilution with SAKUMA Antibacterial Determiner (MIC test). The disk diffusion test and the vitek test were applied to detect ESBLs carried by E. coli.3. Class 1 integrase gene (intI) of 180 E. coli clinical isolates was detected by polymerase chain reaction (PCR) with suitable primer. The drug resistance difference between the positive and negative isolates carrying class 1 integron by the statistical method and the correlation of drug resistance with ESBLs and class 1 integron was analyzed.Results1. The positively detecting rate of E. coli from the clinical specimen was 17.4%, mainly from the Emergency Department (27.6%), the General Surgery (13.8%) , and the Endocrine Department (9.9%) . The higher detection rate's samples Sputum, urine and residue from wound had higher positively detecting rate (25.4%, 22.1 %, and20.4%).2. The resistance rates of 180 E. coli clinical isolates to AMP, quinolones, GM, TMP-SMZ, CFZ, SAM, TOB were 92.3%, more than 70%, 69.1%, 65.2%, 61.3%, 54.7%, and 54.1%, respectlvely. Low resistance rates of E. coliwere observed to IMP (6.6%) , CAZ (3.9%) , PIP/TAZ (3.3%) , and FUR (3.3%) .3. There were 55% of isolates to produce ESBLs in 180 E. coli, with higher resistance, comparied with the ESBLs negative isolates. The 64.4%(116/180) E. coli were positive isolates with class 1 integron, which showed multiple resistance againstβ-Lactams , Quinolones , and aminoglycosides.