Effects Of PLGA-inorganic Composite Biomaterials On The Proliferation And Differentiation Of Mouse Tooth Germ Cells And Postnatal Human Dental Pulp Stem Cells

Tooth loss due to periodontal disease, dental caries, trauma, or a variety of genetic disorders continues to affect most people adversely at some time in the lives. A biological tooth substitute that could replace lost teeth would provide a vital alternative to currently available clinical treatments.To pursue biological tooth and suit to its shape, I compared four different biomaterials,namely PLGA, PLGA/HA, PLGA/TCP, PLGA/CDHA,on the proliferation and differentiation of mouse molar tooth germ cells and postnatal human dental pulp stem cells(DPSCs). The content and results were as follows:A. by cell activity (MTT assay),the results showed that to Hela cells all the cell cytotoxicity degree of biomaterials were zero degree.To DPSCs,the four different biomaterials were deleterious,and PLGA/CDHA was the most;B.after tooth germ cells-biomaterial composites cultured on the renal sac of mice for six weeks,we found the proliferation of cells inside the PLGA/HA was highest of all,and the PLGA was the lowest;while the DPSCs-biomaterial composites, the proliferation of cells inside the PLGA/HA(200um),PLGA/CDHA(320um),PLGA/TCP(320um)was almost the same, and the PLGA was the lowest;C.von kossa staining of tooth germ cells-biomaterials and DPSCs-biomaterials composites cultured for different time displayed a significant amount of mineral in PLGA/TCPand PLGA/CDHA composites;D. the adsorption of cells of PLGA/HA was the best of the four biomaterials. And we found half-baked tooth structure in the cells-PLGA/CDHA and cells-PLGA composites cultured on the renal sac of mice for six weeks;E. we could find integrated tooth structure in the tooth germ cells-biomaterials composites cultured on the renal sac of mice for six weeks.In summary, PLGA/HA and PLGA/TCP were useful for cells to proliferate, PLGA/TCPand PLGA/CDHA are useful for cells to differentiate,while PLGA was not only unuseful for cells to proliferate but also unuseful for cells to differentiate.