| The first departmentObjective: To study the clinical therapeutic effect of Danggu- ishaoyaosan and Tongxieyaofang on diarrhea-irritable bowel syndrome.Method: The case-control study was adopted. The selected cases were randomly divided into two groups according to"Randomized Number Scale": 66 patients in treatment group were administered with Dangguishaoyaosan and Tongxieyaofang; 54 patients in control group were administered with Dicetel. All patients were treated for four weeks. Clinical symptoms before and after treatment were detected respectively to evaluate the therapeutic effects.Result: (1)Clinical symptom therapeutic effect: In treatment group the total efficiency was 90.91%,which was higher than that of control groupIn control group the total efficiency was 77.78%. There was a significant difference between two groups(P<0.05)(.2)Compared the clinical symptom accumulated points before and after treatment: the clinical symptom accumulated points after treatment of the two groups were decreased.The accumulated points descent amplitude on treatment group was much more higher than control group(P<0.05).(3)Compared the main clinical symptom before and after treatment: There was a significant difference between treatment and control group on accumulated symptom points(P<0.05).In treatment group all main clinical symptoms were markedly improved after treatment but in control group only the symptom of pain and diarrhea were makedly improved(P<0.05) .(4)Evaluate medicine side effect and therapeuti- c safety in two groups: there was only three patients on control group occured nausea or had dizziness and headache . There was neither obvious changes in blood, urine ,stool rules before and after treatment nor in Electrocardiogram and liver-renal function. In the course of the treatment, all patients had no undesirable response, which pointed that both the two groups have no toxic and side effect.Conclusion: This clinical research indicates that DangguiSha- oyaosan and Tongxieyaofang can improve patient's clinical symptom distinguishly, which has significant difference from Dicetel .This finding authenticated the clinical utility and feasibility of Dangguishaoyaosan and TongxieYaofang and hinted that chinese medicine has conspicuous ascendency on this disease .Then deep study and approach are deserved .The Second DepartmentObjective: To explore mechanism of Dangguishaoyaosan and TongxieYaofang in curing experimental rat model of IBS by constructing animal model and observing correlated indexes. The aim is to provide experimental evidence for clinical research. Method: 40 healthy male wistar rats were randomly divided into five groups, each group had 8 rats. Diarrheal rats were established by intragastric administration with senna apozem and restraint-stress. 2 weeks later when the models were established, the amount of dried medicinal herb given to rats in large dose group and low dose group was 30g/kg weight and 15g/kg weight respectively. The amount of Dicetel given to control group was 30mg/kg weight. The normal saline was given to normal group and model group respectively. Two weeks later, all rats were killed to observe following index:,weight, motilin(MOT),vasoactive intestin -al polypeptide (VIP),Somatostatin (SS), the driving rate of carbon powder in small intestine and pathology of bowel tissue.Result:(1) Weight change of rats: when models were established the weight of rats in models were decrease,and there was a significant difference between normal group and the others, after treatment there wasn't significant difference between model groups and normal group in the weights of rats (2) Determination of the driving rate of carbon powder in small intestine: Through calculating the length rate of carbon power drivened by bowel we found that model group was hyperkinesia. The driven rate of carbon powder was much higher than Dicetel group, lower dose group and large dose group, but it was the most manifestation in large dose group.(3) Level of MOT,SS,VIP in low dose group, large dose group and Dicetel group was markedly decreased than that of model group(P<0.05),while the VIP level difference between the treatment groups was significant(P<0.05). The level difference of MOT and SS were not significant. (P<0.05).(3)Observation of colonic mucosa tissue: the HE tincted microtome section of rats in five groups are normal ,and there wasn't significant difference between them.Conclusion: This study indicated that bondage and stress suscitate can provoke the intestine's hypermotility.Dangguishaoyao -san and Tongxieyaofang is able to regulate the level of MOT,SS, VIP.This kind of gut hormone can adjust the movement of bowel and the disorder of Water-Electrolyte. It indicated there were different therapeutic effects on different dose groups. it was the most manifestation in large dose group.
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