Research On Effect Of Injecting Gel Into Lung And Locally Immune Response

Objective: By observing absorption and security of selected kinds of onefold gel medium and analyzing absorption, proper dosage, kickback and local or systemic immunity effect of gel plus Mycobacterium Vaccae Vaccine in the rabbit, new evidence could be set up that gel plus immune protein preparation could be used for curing tuberculosis in clinic.Methods:45 long ear rabbits are randomly divided into two big groups: the experimental gel group (20 rabbits) and the immune stimulative group (25 rabbits). Divided 20 rabbits of the group of experimental gel into 4 groups random and there are 5 rabbits in each group:(1)control group, without any disposal(2)a-gel group, injecting a-gel into left lung of long ear rabbit with branchofiberoscope(3)g-gel group, injecting g-gel into left lung of long ear rabbit with branchofiberoscope(4)k-gel group, injecting k-gel into left lung of long ear rabbit with branchofiberoscope. After one week, testing hemic routine and T lymphocyte subsets and observing bowels, especially lung, by anatomizing. And histopathological examination of the local lung injected gel is done. Divided 25 rabbits of immune stimulative group into 5 groups random and there are 5 rabbits in each group:(1)1^st group, that is control group, weekly injecting physiological saline into left lung of long ear rabbit with branchofiberoscope, total four weeks;(2)2^nd group, weekly injecting 5.63μg Mycobacterium Vaccae Vaccine plus 2.5ml k-gel and 0.5ml medical water into left lung of long ear rabbit with branchofiberoscope, total four weeks;(3)3^rd group, weekly injecting 11.25μg Mycobacterium Vaccae Vaccine plus 2.5ml k-gel and 0.5ml medical water into left lung of long ear rabbit with branchofiberoscope, total four weeks;(4)4^th group, weekly injecting 22.50μg Mycobacterium Vaccae Vaccine plus 2.5ml k-gel and 0.5ml medical water into left lung of long ear rabbit with branchofiberoscope, total four weeks;(5) 5^th group, weekly injecting 28.13μg Mycobacterium Vaccae Vaccine plus 2.5ml k-gel and 0.5ml medical water into left lung of long ear rabbit with branchofiberoscope, total four weeks. 2^nd group, 3^rd group, 4^th group and 5^th group are belonged in attacked group. All groups are killed and anatomized after 4 weeks . Testing hemic routine after injection; testing T lymphocyte subsets andγ–INF in blood before and after immune stimulative. Observing bowels, especially lung, and skiving certain pulmonary organization to testγ–INF after anatomizing . Hereinbefore each group is measured animal heat every day and avoirdupois every week .Result: 1 the experimental gel group: animal heat, avoirdupois, hemic routine and T lymphocyte subsets of a-gel group, g-gel group, k-gel group didn't show significantly diversity by comparing with animal heat , avoirdupois , hemic routine and T lymphocyte subsets of control group(P>0.05). Percent of CD₃,CD₄,CD₈ in blood after injection didn't show significantly diversity by comparing with before anatomizing in a-gel group, g-gel group and k-gel group(P > 0.05). Histopathological examination showed a-gel group's, g-gel group's and k-gel group's bronchiole didn't obviously changed by comparing with control group's. All gel groups'alveolus had complete structure and spacing of alveolus was not incrassation and phlogistic cell didn't be found.2 immune stimulative group: animal heat , avoirdupois and hemic routine of 2^nd group, 3^rd group, 4^th group and 5^th group didn't show significantly diversity by comparing with animal heat , avoirdupois and hemic routine of 1^st group(P>0.05). Percent of CD₃,CD₄ of 4^th group and 5^th group showed significantly diversity by comparing with percent of CD3,CD4 of 1^st group(P<0.05). Percent of CD3,CD4 of 4^th group and 5^th group were higher than percent of CD3,CD4 of 1^st group. Percent of CD₈ of 3^rd group, 4^th group and 5^th group showed significantly diversity by comparing with percent of CD8 of 1^st group(P<0.05). Percent of CD8 of 3^rd group, 4^th group and 5^th group were lower than percent of CD8 of 1^st group. Content ofγ–INF of local lung, in which was injected k-gel plus high dosage Mycobacterium Vaccae Vaccine, of 4^th group and 5^th group increased but content ofγ–INF of other groups didn't change. By comparing with 1^st group histopathological examination of 4^th group and 5^th group showed bronchiole didn't obviously changed and alveolus had relatively complete structure. But spacing of alveolus was incrassation and lymphocyte was found in spacing of alveolus.Conclusion: 1 a-gel, g-gel and k-gel have good fluidity and conglutination and can satisfying and well-proportionedly mixed with dissolved Mycobacterium Vaccae Vaccine.2 Security of a-gel, g-gel and k-gel was validated elementarily. When a-gel, g-gel and k-gel were injected into rabbit's lung with branchofiberoscope, three kinds of gel can be adequately absorbed within one week. A-gel, g-gel and k-gel can be used in clinic as selective gel which can be injected into lung by branchofiberoscope.3 Immune protein preparation is absorbed by locally pulmonary organization in security when k-gel was injected into rabbit's lung with branchofiberoscope.4 Locally immune stimulative can cause systemic immunoreaction in healthy rabbits.