| Objective: In this study, we analyzed the expression of PTTG(Pituitary Tumor Transforming Gene)? PTEN(phosphatase and tensin homology deleted on chromosometen) protein and the relationship between them in gastric cancer. We also analyzed the relationship between the expression of PTTG,PTEN and the survival rate of patients with gastric cancer. We want to evaluate its prognostic value to gastric cancer, helping the detection of disease and clinical treatment.Methods: We collecte 32 gastric cancer tissues, 38 the brims of gstric carcinomas and 36 normal tissues-the distanse between the normal and the cancer tissue is about 5cm.The paraffin-embedded section of all the tissues was cut by surgical operation of the second hospital of HeBei Medical University. All the patients did not accept radio-therapy, chemical-therapy and hormone immunity therapy.The diagnosis of the pathology was explicit,and the clinical information was complete.32 male and 6 femal, the age is 39~72 years old, median age is 55.5 years old. Pathology, stage and the condition of lymph node metastasis were appreciated by pathology director. We calculated the expression of PTTG and PTEN with immnohistochemistry(PV) and the analysis of the double-core sort in the positive cells, and studied the relation between PTTG and PTEN and the clinic pathology's parameter of gastric carcinomas. Statistics handling used SPSS13.0 software. We used the Chi-Square test and Fisher exact probabilities for the comparision of the expression in every tissue and the relation between PTTG? PTEN and the clinic pathology's parameter of gastric carcinomas.It used the spearman test for the relation between PTTG and PTEN of gastric carcinomas. The impact of PTTG and PTEN on the survival rate of patients used survival analysis of Kaplan-Meier. P<0.05 is regard as having differences.Results: 1 The expression of PTTG in the different tissues: The expression of PTTG is 75.00%(24/32)in the gastric carcinomas, 60.53%(23/38)in the brim of gastric carcinomas, 19.44%(27/36) in the normal tissues. In this three tissues,the expression of PTTG has distinct difference (P<0.05). Between the gastric carcinomas and the brim of gastric carcinomas, the expression of PTTG has not distinct difference (P>0.05). Between the gastric carcinomas and the normal tissues, the brim of gastric carcinomas and normal tissues, the expression of PTTG has distinct difference (P<0.05).2 The expression of PTEN in the different tissues: The expression of PTEN is 8.12%(9/32)in the gastric carcinomas, 61.58%(24/38)in the brim of gastric carcinomas, 88.89%(32/36)in the normal tissues. In this three tissues,the expression of PTEN has distinct difference (P<0.05). Between the gastric carcinomas and the normal tissues, the brim of gastric carcinomas and normal tissues, the expression of PTTG has all distinct difference (P<0.05).3 The relation between PTTG and gastric cancer clinicopathological parameters: The expression of PTTG has not distinct difference (P>0.05) in the age, gender, histological type, location of tumor and tumor size; But high expression of PTTG closely related to the the depth of invasion, lymph node metastasis, distant metastasis, UICC stage of gastric cancer(P <0.05).4 The relation between PTEN and gastric cancer clinicopathological parameters: The expression of PTEN has not distinct difference (P>0.05) in the age, gender, histological type and location of tumor; But high expression of PTEN closely related to the the tumor size, depth of invasion, lymph node metastasis, distant metastasis and UICC stage of gastric cancer(P<0.05).5 The relationship of PTTG and PTEN: There is not correlation between PTTG and PTEN (P>0.05).6 The relation between the expression of PTTG,PTEN protein and prognosis of gastric cancer: We followed-up 28 patients with complete clinical information in 2 to 42 months, the median follow-up time was 25 months. Kaplan-Meier survival analysis showed that: The survival rate of patients with positive expression PTTG is significantly lower than that the patients with negative PTTG (P<0.05), and the survival rate of patients with negative expression PTEN is significantly lower than that the patients with positive PTEN (P<0.05).Conclusion 1 The expression of PTTG and PTEN in gastric cancer tissues, the brim of cancer tissues and relatively low in normal tissues is significant differences, which shows that the PTTG and PTEN involved in the occurrence of gastric cancer in the early stage of carcinogenesis, and may have a certain sense for early gastric cancer.2 The relation between PTTG,PTEN and gastric cancer clinicopathological parameters: The expression of PTTG and PTEN is closely related to the the depth of invasion, lymph node metastasis, distant metastasis, UICC stage of gastric cancer(P <0.05). This study show that the expression of PTTG and PTEN is a reflection of occurrenc,invasion and metastasis potential of gastric cancer, and they are important prognostic markers for the early gastric cancer,and have a potential relationship to the prognosis of patients.3 The relation between expression of PTTG and PTEN in gastric cancer tissues has not distinct difference (P>0.05). PTTG and PTEN may play a role alone in the development of gastric cancer. The Oncogene and the tumor suppressor gene mey have a certain relation, which need further studied.4 The survival rate of patients with positive expression PTTG is significantly lower than that the patients with negative PTTG,and the survival rate of patients with negative expression PTEN is significantly lower than that the patients with positive PTEN. Therefore the expression of PTTG and PTEN can affect the survival rate of gastric cancer patients ,and PTTG and PTEN can be used as molecular markers to determine the prognosis of gastric cancer.
|
PDF Full Text Request