Primary Screen Of Active Sites Extracted From Actinidia Chinensis

Actinidia chinensis is of high pharmacological activity. Clinical practices have showed that radix of Actinidiae chinensis is effective in the treatment of the cancers of digestive system and hepatitis B. Much study on chemical composition and anti-tumor of radix of Actinidiae chinensis have been done in the modern pharmaceutical. No study on screening anti-HBV or anti-tumor active site of radix of Actinidiae chinensis has been reported up to now, it is enormously confined at the place of its clinical application.[OBJECTIVE]To identify anti-tumor, antiviral active site and chemical composition category of radix of Actinidiae chinensis through pharmacological experiments and to pave a solid basis for further development of radix of Actinidiae chinensis.[METHODS] 1. Radix of Actinidiae chinensis were extracted by the methods such as reflux, column chromatography and thin layer chromatography. 2. Bel-7402,SW-620,MDA-MB-231 cell lines were selected for screening anti-tumor active site by MTT, growth curve and cell apoptosis dyeing. 3. Antiviral active site were screening by MTT and detection of HBsAg, HBeAg.[ RESULTS ] Y₁+Y₂, A₁+A₄+Y₁, A₁+A₃+A₄ and A₁+A₄+A₆ were determined effective by MTT colormetric assay and growth curve.They were of strongly inhibitory action to three tumor cells in vitro and dose dependent. Tumor cell apoptosis could be induced by A₁+A₄+A₆, A₁+A₄+Y₁. Contents of A₁+A₄+A₆ was 54.35%, Contents of A₁+A₄+Y₁ was 62.64%. The treatment index of A₁+A₃+A₄, Y₁+Y₂ on HBeAg, HBsAg exceeded 2. It implied the two sites inhibited HBeAg, HBsAg. Contents of A₁+A₃+A₄ was 60.28%. The two sites above are chemically determinded as triterpenoid saponin by foam tests and Liebermann-Burchard reaction.[CONCLUSIONS] The results suggested that A₁+A₄+A₆, A₁+A₄+Y₁ and A₁+A₃+A₄ were the anti-tumor active site and the antiviral site of Radix of Actinidine chinensis, respectively. The mechanism of action was related to induction cell apoptosis. The active site affected little to normal cell. It would provide a basis for further isolating composition of antitumor and antiviral.