The Recognition Of Basophil In Chronic Myeloidleukemia

ObjectiveIt is well known that basophils of the abnormal in patients with chronic myeloid leukemia in the bone marrow and peripheral blood has become indicators of poor prognosis. At the same time, interferon-αis the validity treatment in the chronic myeloid leukemia. so the plasmacytoid dendritic cell(pDC) - interferon professional producing cells have become the issue between domestic and international research workers. Experimental animal studies have found that simply identified by the cell morphology on the basophils do not have molecular phenotype by the current international identified. Well, chronic myeloid leukemia in patients with abnormal number of basophils is the true sense of basophils? What is the relationship between basophils and plasmacytoid dendritic cells? This is the significance of this research.MethodAfter inspection of Hematology, bone marrow, chromosome and molecular, a clear diagnosis of chronic myeloid leukemia in chronic phase, interferon-αeffective treatment, interferon-αtreatment ineffective accelerated phase or blast crisis phase of patients will be divided into the three groups, using Ficoll density gradient centrifugation from the three patients with bone marrow mononuclear cells, and then use proceeds of Ficoll-Hypaque morphology and significant "basophils " separation of smear staining, inverted microscope and light microscope observation of cell morphology, for cell counting basophils of a statistical test. Then, use of the FcεRI monoclonal antibody beads for magnetic activated cell sorting to further purified cells . Will be followed by plasmacytoid dendritic cell phenotype to flow cytometry, compared three groups of patients in the so-called "basophils " have plasmacytoid dendritic cell phenotype ratio statistics do examination judged significant.ResultsCML-MNC After Ficoll-Hypaque separation, basophils purity of more than 90 percent, Wright - Giemsa staining of blood smears, basophils appear as mononucleated cells of 10 - 14μm diameter, with a nucleus containing condensed chromatin. This nucleus, said in "clover" or in "brioche, " contains lobes, separated by fissures, generally sealed at the extremities, resulting in a round or oval global appearance. The cytoplasm is filled with many round or oval, purple, dark granules of variable size, partially covering the nucleus. The radical change of interferon treatment group or invalid "basophils " counting period of significantly chronic group and interferon treatment were significantly more effective. Flow cytometry to detect cell surface markers, in which morphological that "basophils " has found a certain amount of expression of cell surface markers pDC, and the signs of past and also identified by basophils there is a clear sign of the difference. And with the progress of the disease and treatment of the changes in the expression of cell surface markers pDC were significantly reduced. Different disease progression period, the number of different, the difference is statistically significant (P <0.05).Conclusion1. Chronic myeloid leukemia in patients with isolated from the morphological that "basophils " the percentage of content with the progress of the disease increased, with the progress of effect reduced.2. Chronic myeloid leukemia in patients with isolated from the morphological that "basophils " and some have pDC cell surface markers. However, the "pDC" content with the progress of the disease reduced, with the progress of effect increased.3. chronic myeloid leukemia in patients with isolated from the morphology that the "basophils " likely to be part of the true sense of the basophils, pDC , and the other part of cells has not yet been recognized. However, needing further research and discovery.