Effect Of PI3K/AKT Pathway On Growth And Adherence Of Human Hepatoma Carcinoma Cells

AIM:To investigate the effect of phosphoinositide 3-kinase(PI3K)- protein kinase B(AKT) pathway on the growth,Adherence and invasion of human hepatoma carcinoma cell linc SMMC-7221 and its mechanism.METHODS:MTT reduction assay was used to evaluate the inhibitory of SMMC-7221cells treated by LY294002(5,10,20,40umol/L)for 24h,48h,72h,96h.The tumor adhesion ability was detected by cell-matrix adhesion experiment.Flow cytometry(FCM)detect CD44S expression.Immuncytochemistry was used to detect the expression of VEGF,CD44V6 in SMMC-7221 cell lines.RESULTS:LY294002 at concentrations of 5-40umol/L could inhibit the SMMC-7221 cell proliferation in time and dose dependent manner;5,10umol/L LY294002 made VEGF,CD44V6,CD44S expression degression compared to control group(CD44S:42.84±6.35,20.21±4.5 VS 89.23±3.91,P＜0.01;VEGF:103.11±18.60,68.99±15.99 VS 137.84±22.50,P＜0.01;CD44V6: 47.33±6.15,33.09±5.23 VS 61.36±7.39,P＜0.01);5,10umol/L LY294002 made SMMC-7221 adhesion ability breakdown compared to control group(0.498±0.024,0.407±0.029VS 0.616±0.080,P＜0.01).CONCLUSION:PI3K/AKT pathway is very important in the SMMC-7221 growth,adherence and invasion,its catastaltic may make very importance in the management of hepatocellular carcinoma.