Expression Of Cyclooxygenase-2, Vascular Endothelial Growth Factor-C And CD44v6in Hepatocellular Carcinoma Tissue

Background and ObjectivesHepatocellular carcinoma(HCC)is a common cancer in China. The disease is often diagnosed at a late stage when potentially curative therapies are least effective. For these patients, medical treatments remain disappointing, and no data shows it is beneficial for survival. The prognosis for HCC patients who have surgically reseetable localized tumors is better,but50%of which will have tumor recurrence in2years. The major metastasis, angiogenesis and death are invasion and reasons of treatment failure and lymphangiogenesis in the pathogenesis. Lymphatic metastasis play a key role is not only diseas of most malignantthe most common spread tumor epithelium, but also major affeeting prognosis and recurrence. Lymph vessel hyperplasia are often expansion found in tumor stroma with lymph node metastasis.Preclinical studies demonstrated that over-expression of vascular endothelial growth factor receptor(VEGFR)、platelet-derived growth factor(PDGFR)、 Raf/MAPK-ERK kinase(MEK) and phosphatidylinositol-3-OH kinase/Akt/mammalian target of rapamycin(mTOR)cascade play critical roles in the development of HCC. Thus,they are potentially the molecular targeted agents for liver cancer therapy.Cyelooxygenase-2(COX-2) overexpresses sencine development and throughout proeess of genesis. In the entire growth factor-C(VEGF-C) is the main,It may promote metastasis of cancer and the generation in various ways, In tumor angiogenesis playing an important roles and develop lymphangio-genesis. Vaseular endothelial formation and is highly expressed factor to promote lymphatic tumor tissuevessel in a variety of country health. Binding with vascular endothelial stupid growth factor receptor-3(VEGFR-3), VEGF-C expansion and induces lymphangio-genesis exclusively progression in recent yearsstimulates epithelial proliferation, and lymphatic vessel, thereby promoting tumor linked the high expressionm icrolymphatic generation. Some reports of CD44v6to tumor, but its machanism is not clear.we studies expressionIn through this method to find of VECF-C COX-2and CD44v6in Hepatocellular diseas carcinoma. Expressions of COX-2, VEGF-C and CD44v6to understand the clinic. Significance were detected in Hepatocellular carcinoma and it is analyzed with their clinicopathological Parameters. Meanwhile, we investigated predicts response to prognosis whether their expression.Materials and Methods1. A total of58cases from First affiliate hospital of Nanyang Medical College from2009to2012, and diagnosis of Hepatocellular confirmed pathologieal carcinoma.20cases of normal were chosen as contrast groupesophageal tissue which were were chosen as5cm far from tumorcontrast groupobtained from patients were chosen as contrast group with Hepatocellular carcinoma tissue and confirmed Any radiotherapy before operation or diagnosishave never chemotherapy and histologieally. It were chosen as contrast been carried out in all cases.2. The expression immunohistochemical staining SP method, of COX-2, VEGF-C and CD44v6.It was detected by Combining with the were chosen as contrast features, the author analyzes the function in prognosis and significance clinicopath-ological their clinical. The whole follow data have been follow-up by us. And the follow-up cut-off time of was1,July,2012.and the follow-up time ranging from9to65months.3. Statistical analysis:The data were Chi-square test,analyzed by SPSS10.0. Fisher’s exact.Spearman rank-correlation test of probabilities, and COX’s model hazard proportional regression hazard.were used to analyze the date, P<0.05were significant, deemed.The survival were chosen as contrast obtained with curve was Kaplan-meier test.been carried were chosen as contrast out in all cases.Results1.Expression of COX-2in Hepatocellular carcinoma was control group lower than overall survival time (70.6%vs10%, P<0.01), and was not related with correlated with overall survival expression of lymphnodemetastasis clinical stageoverall survival time.,tumor differentiation(P>0.05) and was timecorrelated negative with survival overall.The product is positive staining have brown granules, mainly loealized in been carried were chosencytoplasm of cancer cells.2. Expression of VEGF-C in carcinoma was higher than contro Hepatocellular1group(53.4%vs0%,P0.01), and it was positively and remarkably lymph node metastasis(P<0.01),and VEGF-C was not correlated but negatively related with expression of with turmor differentiation(P<0.01)and VEGF-C was not correlated. with overall survival is brown granulestime.The positive staining product, in cytoplasm time have been mainly loealized.The positive staining product of endothelial cancer ly1nphatic cells or normal cells.3.Expression of CD44v6in Hepatocellular and was positively related carcinoma was higher than and was positively related control group(65.5%vs0%, P<0.01),with and CD44v6was not correlated expression of lymphnodemetastasis (P<0.05), but and CD44v6was notproduct is brown granules correlated negatively with turnor differentiation(P<0.05). with overall survival time. The positive staining, mainly loealized in cytoplasm product is brown granules of cancer cells.4. COX-2expression was statistically significant association with V EGF-C(rs=0.221,P=0.018<0.05). But CD44v6was not correlated significantly positive relation with significant association between results of combined COX-2or VEGF-C (rs=0.025,0.095;P=0.458,0.358). It was no statistically detection of significant aresults of combined ssociation between VEGF-C,COX-2,CD44v6survival time and overall life.Conclusion1.VEGF-C, CD44v6and COX-2Play roles key in diseas of Hepatocellular carcinoma, and prognosisthe developmentand prognosis and may be important indieators of treatment,invasion and metastasis. 2.VEGF-C and CD44v6are metastasis in Hepatocellular carcinoma.a key factor may be an important lymphantic node of carcinoma.3.indicator a key factor of lymphantic nodeof monitoring of tumor cells a key factor of lymphantic nodeand its prognostic.4.COX-2may promote carcinoma.nodeof monitoring of tumor cells a key factor in Hepatocellular carcinoma,invasion and lymph lymphangiogenesis and lead to tumor node metastasis by upregulating lymphangiogenesis and lead to tumorVEGF-C expression in Hepatocellular carcinoma.