Expression Of Foxp3 MRNA In Peripheral Blood Monocytes Of Patients With Hepatitis B

Objective: To explore the expression of Foxp3 mRNA in the peripheral blood monocytes (PBMCs) of patients with HBV infection, and its relationship with lapsing to hepatitis B virus infection. The serum level of IL-10 and TGF-βwas examined by ELISA, and their relationship with Foxp3 mRNA.Methods: Real time reverse transcription-polymerase chain reaction (RT-PCR) was used to examine Foxp3 mRNA expression in the PBMCs from 19 cases with acute hepatitis B, 50 cases with chronic hepatitis B, 21 cases with cirrhosis post hepatitis B, 15 cases with chronic severe hepatitis B, and 15 cases normal controls. The serum level of IL-10 and TGF-βwas examined by ELISA, and their relationship with Foxp3 mRNA.Results: Foxp3 mRNA levels of acute hepatitis B, chronic hepatitis B, liver cirrhosis, chronic severe hepatitis B are higher than normal control group (P<0.05), Foxp3 mRNA level of severe hepatitis is higher than those of other groups (P<0.05); IL-10 and TGF-βlevels of HBV infection groups are evidently higher than those of normal control group (P<0.05), also, it is evidently higher in acute hepatitis B than in chronic hepatitis B of IL-10 (P<0.05); TGF-βlevels of acute hepatitis B is no statistical difference between liver cirrhosis and chronic severe hepatitis B (P > 0.05), and there is statistical difference between other groups(P<0.05). There is no statistical relevance between the levels of IL-10, TGF-βand Foxp3 mRNA levels.Conclusion: Foxp3 mRNA levels of acute hepatitis B, chronic hepatitis B, liver cirrhosis, chronic severe hepatitis B are higher than normal, Foxp3 mRNA level of severe hepatitis is higher than those of other groups . Foxp3 mRNA expressions in the PBMCs may be involved in the pathogenesis and closely correlated with the prognosis of HBV infection. There is no statistical relevance between the levels of IL-10, TGF-βand Foxp3 mRNA levels.