| The objective of this study is to observe the expression and clinical significance of the human microRNA 122 in liver carcinoma, and investigate its target molecule and function. The subject is divided into two parts.The first part:To observe the expression and clinical significance of the human microRNA 122 in liver carcinoma by real time flurescent quantitative RT-PCR. We developed a specific, sensitive, reproducible, rapid, accurate and full-sealing real time flurescent quantitative reverse transcriptase polymerase chain reaction (RT-PCR) method. The expression of has-miR-122 was detected in 70 samples of liver carcinoma tissues and those adjacent ones. Compared to the adjacent tissues, the expression of miR-122 is significantly reduced. The low expression of miR-122 is related the neoplasm differentiation degree and liver fibrosis. So it may be look as a new diagnostic and prognosis index.The second part:With Pictar and Targetscan software, laminin y 1 may be a target molecule of miR-122. There are two possible pairing positions located in 2490-2496 and 2331-2337 of LAMC1. We constructed miR-122 expression vector and synthesized anti-miRNA oligonucleotides of miR-122(AMO-122). In isolated HSC, it showed lose-of-function and gain-of-function by semi-quantitative western blot. The expression of miR-122 is negatively associated with LAMC1.Our results investigated the expression of miR-122 and its relationship of LAMC1. Our study will contribute to better understanding of the mechanism of liver carcinoma and be helpful to development of new approaches for cancer therapy.
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