Expression And Significance Of Bad In The Pulpy Nucleus Tissue Of Degenerated Lumbar Discs

Objective: Through the detection of the expression level of bad in pulpy nucleus of human degenerated lumbar intervertebral discs, to investigate the roles of bad in disc degeneration at Lumbar Disc Herniation tissue,and to understand the pathology of Lumbar Disc Herniation(LDH).This may provide an experimented proof for the diagnosis of clinical Lumbar Disc Herniation(LDH).Methods: 1.The fresh pulpy nucleus tissue was obtained surgically and were fixed in 10% formalin , imbedded in Paraffin and sliced by 4μm.2.The degeneration of pulpy nucleus tissue was observed with HE staining. 3. The expression of bad in experimental group (40 fresh pulpy nucleus of patients with lumbar disc herniation, 27 males and 13 females) and control group (8 fresh pulpy nucleus of patients with idiopathic scoliosis, 3 males and 5 females) were detected with TdT-mediated dUTP-biotin nick end labeling(TUNEL) and immunohistochemical techniques. 4.The results were analysed with SPSS 11.5 for windows.Results: 1.Histopathologic examination of herniated intervertebral disc tissues showed granulation tissue at the peripheral portions of herniated discs.2.The average apoptotic index(AI)in degenerative lumbar intervertebal disc was 63.51,which was significantly higher than that of normal cases(20.12)(P<0.01).3.bad expression was significantly increased in patient group. The stronger bad protein expressed,the bigger the apoptotic index was(P<0.01).Conclusions: There is a significantly increasing expression of the bad and increasing apoptosis in the degenerative lumbar intervertebral discs.Bad protein take part in the occurrence and progression of lumbar intervertebal disc degeneration.