The Change Of The High Concentration Etomidate For Mitochondrial Membrane Potential In HL-60 Cells

Objective:Choose the apoptosis of HL-60 cells as the research object, using the fluorescence probe of JC-1 to observe the change of mitochondrial membrane potential in the apoptosis of HL-60 cells, and using the fluorescence probe of Calcein AM to detect the opening of the mitochondrial permeability transition pore.Methods:HL-60 cells were cultured at a concentration of 1.5×105/ml. Two groups were processed in accordance to different methods. The control group was blank (0μM etomidate for 24h); the injury group was treated with 500μM etomidate for 24h. After collecting the cells, using the fluorescence probe of JC-1 by flow cytometry to detect the change of mitochondrial membrane potential and the fluorescence probe of Calcein AM to detect the opening of the mitochondrial permeability transition Pore.Results:The experimental result in the detection of mitochondrial membrane potential by fluorescent JC-1 show that the proporation of green fluorescein cells and all in the injury group(41.72%)is obviously higher than the control group(3.53%); the experiment of the mitochondrial permeability transition pore opening show the mean fluorescence intensity in injury group (3.54) is obviously lower the contral group(33.71).Conclusions:One of the way the high concentration etomidate induce the apoptosis of HL-60 cells is through activating the opening of the mitochondrial permeability transition pore and changing the mitochondrial membrane potential.Combination JC-1 with flow cytometry is ideal method to detect mitochondrial membrane potential.