Effect Of Containing Jin Dan Shui Wan Solution Serum On BMP-2, ER Expression Of Rat Bone Marrow Stromal Cells

Objective: To further study of osteoporosis associated with hyperlipidemia; research of high cholesterol and serum's Jin Dan Shui Wan-containing serum intervention rat bone marrow stromal cells BMP-2, ER mRNA expression changes.Methods: adult rat femur by density gradient centrifugation separation of bone marrow stromal cells, cultured cells to the third generation, and was identified by flow cytometry. Produce third-generation cell, were randomly divided into normal serum control group: two percent blank serum (volume ratio); high serum cholesterol injury Group: final concentration of 4 mmol / L, high serum cholesterol; medicine + pretreatment serum high serum cholesterol injury group: 2% serum Chinese medicine (volume ratio) after pretreatment 2 hours + final concentration of 4 mmol / L, high serum cholesterol. Total RNA was isolated from cells recovered. Detection and ER BMP-2 mRNA expression changes. And analyzed statistically.Results: 1. Blank serum compared with the control group, high serum cholesterol cultured bone marrow stromal cells proliferation was inhibited. BMP-2 bone marrow stromal cells, the ER mRNA and protein expression levels of(p<0.01) 2. Joining Jin Dan solution containing serum protection Shuiwan two hours later to join a high serum cholesterol group, the cell growth conditions improved significantly, BMP-2, ER mRNA and protein levels than pure high serum cholesterol increased expression of the injured group(p<0.01).Conclusion: Solution's Jin Dan Shui Wan-containing serum can enhance high serum cholesterol environment bone marrow stromal cells BMP-2, ER mRNA expression, significantly improved bone marrow stromal cell growth state is conducive to the treatment of osteoporosis.