The Curative Effect Of HO-1 On The Necrosis Of Heart Cells Induced By Pancreatitis Associated Ascitic Fluid.

ObjectiveTo explore the mechanism of acute heart injury in severe acute pancreatitis(SAP) and the conservative mechanism of HO-1 on it through observing the effect of pancreatitis associated ascitic fluid(PAAF) on the necrosis of normal heart cells and the curative effect of HO-1 on the injury.MethodsThe forty rats were established into SAP models by retrograde injection of 5% sodium taurocholate into the pancreatic duct and then collect the PAAF for reservation. Another forty rats were established into perforative peritonitis(PP) models and then collect the perforative peritonitis associated ascitic fluid(PPAF) for reservation. The last forty SD rats were randomly divided into five groups: NS group,PAAF group,PPAF group,PAAF+hemin group and PPAF+hemin group. Serum concentrations of amylase,lactic acid dehydrogenase and creatine kinase were determined at 12th hour post-operation. The expression of HO-1 in the heart of hemin-treated rats was determined by western blot method; all rats were sacrificed to remove herat tissues for analysis of MDA concentration, SOD activity and caspase-3 activity as well as TNF-a contents. In addition, histopathological changes were investigated and cardiac muscle cell apoptosis was detected by TUNEL method.Results There were no statistical differences between SAP groups and PP groups,PAAF groups and PPAF groups,PAAF+hemin groups and PPAF+henin groups. The amylase,lactic acid dehydrogenase and creatine kinase levels of serum and the MDA concentration, SOD activity and caspase-3 activity as well as TNF-a contents of heart tissue in the PAAF groups were higher than PAAF+henin groups, while lower than SAP groups . The amylase,lactic acid dehydrogenase and creatine kinase levels of serum and the MDA concentration, SOD activity and caspase-3 activity as well as TNF-a contents of heart tissue in the PPAF groups were higher than PPAF+hemin groups, while lower than PP groups,and NS group was the lowest, which were identical with heart pathological changes and the apoptosis rate of cardiac muscle cell. However SAP groups and PP groups were the highest. The expression of HO-1 and SOD activity of the heart in the NS groups were higher than PAAF groups, while lower than PAAF+hemin groups, and SAP groups were the lowest. As the former like, NS groups were higher than PPAF groups, while lower than PPAF+hemin groups, and PP groups were the lowest.ConclusionsThe cardiac muscle cell apoptosis and injury could be induced by pancreatitis associated ascitic fluid. The stable heart injury model could be established by PAAF. HO-1 has a potent protective effect on acute heart injury induced by pancreatitis associated ascitic fluid in rats. Induction of HO-1 expression can inhibit the progress of heart damage, which might be due to the alleviation of lipid peroxidation and reduction of caspase-3 activity or inhibition of TNF-αlevel.