Effect Of Heme Oxygenase On Apoptosis And Apoptosis Gene In Hepatic Ischemia Reperfusion Injury In Rats

Hepatic ischemia reperfusion (IR) injury occurred in many courses, such as orthotopic liver transplantation, shock, the operation of clamping hepatic artery and portal vein and so on. The mechanism of hepatic IR injury is elusive, which could be associated with apoptosis, reactive oxygen species, mitochondrial permeability transition and endoplasmic reticulum stress. The studies showed that inhibition of hepatocellular apoptosis could prevent hepatic IR injury. Heme oxygenase (HO) is an initial and limiting enzyme of heme oxidization, which can catalyze the heme into carbon monoxide (CO), biliverdin and free iron. HO is involved in many biological processes including apoptosis inhibition, signal transduction and so on. Recent researches have revealed HO may alleviate hepatic IR injury, improve liver function and lessen hepatocellular apoptosis in rats.Objectsive: The present study was designed to investigate the role of HO-CO system in the pathogenesis of hepatic IR injury in rats by using the HO inducer (hemin) and the selective HO inhibitor (zinc protoporphyrin, ZnPP).Method:...