Altered Activation Of Neuronal Shc In Response To Oxidative Stress In The Rat Hippocampus

Objectives: Research on the expression of rat N-Shc protein; the changes of N-Shc protein phosphorylation of hippocampus in rats after stimulated by H₂O₂ at different time points; the phosphorylated level of N-Shc protein and PKC5 protein of rat hippocampus and changes of the combination of both after stimulated by H₂O₂ in different concentrations. To explore the effects of oxidative stress on the function of N-Shc protein of hippocampus in rat. Methods: Western blot technique to detect:â‘ The expression of She family proteins in rat nervous system and other systems;â‘¡The expression of N-Shc and PKC8 proteins in brains of different month-old rats;â‘¢The changes of hippocampal N-Shc protein phosphorylation after stimulated by H₂O₂ in different time points;â‘£The changes of phosphorylation level of hippocampal N-Shc protein and PKC8 protein and the combination of both after stimulated by H₂O₂ in different concentrations.Correcting the collected Data with the density of normal control as the standard, then ussing SPSS for windows 13.0 software for one-way analysis of variances. Results: The expression of rat N-Shc protein concentrated in the nervous system, almost no expression in heart, liver and muscle tissues; only a small amount of expression of brain N-Shc protein in pregnant 12.5 days and 15.5 days, and the expression increased significantly after birth, but PKCδprotein express in the brain of rats in every stages; phosphorylation level of N-Shc protein of hippocampus in rat rising rapidly in 5 min and continuing for 30 min after H₂O₂ stimulating; Phosphorylation levels of N-Shc protein and PKCδprotein increased gradually after stimulated by H₂O₂ in different Concentration, there is significant difference between group stimulated by 5 mM H₂O₂ and the control group (P<0.01); the combination between N-Shc protein and PKCδprotein increased significantly after stimulated by H₂O₂ in different concentrations, combining weight increasing with the increasion of the concentration of H₂O₂, there is significant difference between group stimulated by 5 mM H₂O₂ and the control group (P <0.05). Conclusion: The N-Shc protein in the hippocampus of rats are Phosphorylated when stimulated by H₂O₂,and the changes of phosphorylation depend on stimulating time and stimulating concentration of H₂O₂; The stimulation of H₂O₂ can promote combination between N-Shc protein and PKC8 protein, and the combination increases with the increasing of H₂O₂ concentration. Our findings suggest that N-Shc protein metergasis under oxidative stress may has a positive role in inhibiting apoptosis.