The Study Of Expression And Significance Of Sterol Regulatory Element Binding Proteins In The Liver Of Patients With Fatty Liver Disease

[Objective]:Observation of SREBPs expression in the liver tissue of patients with fatty liver disease,and the relationship between SREBPs expression and hepatic steatosis,inflammation,fibrosis of liver.Investigation the role of SREBPs in the liver steatosis.Try to provide new methodsto prevent and treat fatty liver diseases.[Methods]:40 cases of patients with fatty liver disease were selected,including 20 cases of non-alcoholic fatty liver disease(NAFLD),and 20 cases of alcoholic fatty liver(ALD).20 cases of the control group were non-activity of HBV infection(ASC) patients with no fatty liver confirmed by liver biopsy.Liver biopsy was carried out after the B-positioning and TSK one-time biopsy needle biopsy method,liver tissue was fixed by 10%formalin,and paraffin-embedded sections of conventional stored.At the same time 15 ml venous blood was collected,10m1 serum separation and keep in -70℃.Hitachi 7060 with automatic biochemical analyzer to measure lipids(TG,CHO),fasting blood glucose(FSG)and serum enzymes(AST,ALT and GGT);electrochemiluminescence Determination of fasting insulin(FINS).Liver tissue dehydration,paraffin-embedded,continuous section(4μm thick),a fiber mesh HE staining,light microscopy observation,detected liver tissue.SREBPs was detected by SP method and compared with control group.[Results]:Compared with the control group,the liver tissue in the patients with NAFLD and ALD group showed diffuse light,moderate and severe hepatic steatosis, serum ALT,AST,TG,CHO,FSG,FINS level were also significantly differences fiom control group(P＜0.05).SREBPs expression in the liver tissue increased significantly(P＜0.05),and showed a positive correlation to degree of fatty change in the liver tissue.The above indicators in ALD and NAFLD group were not significantly different(P＞0.05).[Conclusions]:1.The serum level of ALT,AST,TG,CHO,FSG and FINS increased in patients with Non-alcoholic fatty liver disease and alcohol-induced liver disease.2.SREBPs expression in the liver tissue of patients with alcoholic and nonalcoholic fatty liver disease increased significantly and positively correlated to the degree of fatty change in the liver.3.SREBPs expression in the liver tissues of patients with fatty liver disease result in more fatty change,inflammation and fibrosis in the liver.