Preliminary Studies Of Urinary Metabolic Profile In Diabetes Mellitus In Population Of Uygur And Kazak

Objective:this study aims to determine urinary metabolic profile in diabetes mellitus in population of Uygur and Kazak,and to explore the application of nuclear magnetic resonance spectroscopy-based method to investigate the pathogenesis of diabetes mellitus.Methods:the standard of group division is conducted in accordance with "diagnostic criteria of diabetes mellitus issued by the world health organization in 1999", Urine samples were collected from each volunteer,including the subjects of normal fasting glucose,the subjects of impaired fasting glucose,type 2 diabetes mellitus patients. The urine samples were collected in centrifuge tubes of 50 ml with 1 ml 1%sodium azide, and frozen in refrigerator of-20℃.Preparation of NMR samples:the samples were defrosted in 4℃,aliquots of 550μL urine samples were made up to 600μL with 50μL phosphate buffer(1.5 M,pH 7.47) and any precipitate removed by centrifugation.550μL of supernatant were transferred to 5-mm NMR tubes.NMR spectra were recorded on a Bruker spectrometer at a proton frequency of 500 MHz.Principal components analysis was carried out using SIMCA-P10.0(Umetrics AB,Umea,Sweden).Results:this study demonstrates obvious metabolic urinary differences between T2DM group and NFG group in the two nations,and the metabolites changed were mainly glucose and creatinine. According to the average spectra,there is an increase in taurine,glucose etc.and a decrease in creatinine,hippurate,DMA,acetate,formate in T2DM group of Uygur;the concentration of citrate and lactate in IFG group of Uygur is higher than that of T2DM group.There is a remarkable increase in glucose in T2DM group of Kazak,but minor changes in lactate and citrate.Conclusions:~1H NMR combined with principal component analysis(PCA) is a good research method to investigate the urinary metabolic profile in population of the different degree of fasting blood glucose.