| PART I AN ASEPTIC LOOSENING ANIMAL MODEL[OBJECTIVE] Establish an animal model for aseptic loosening of total joint replacement and quantitatively evaluate the wear-debris induced osteolysis.[METHOD] Air pouches were established on the back of BALB/c mice by injecting sterile air subcutaneously. A section of calvaria from a syngeneic mouse donor was implanted in the air pouch. One group of bone-implanted pouches was stimulated with polyethylene debris, and the remaining bone-implanted pouches received saline alone as controls. 14 days after the implantation, all mice were killed. The air pouches with the implanted calvaria were obtained for molecular and histological analyses. [RESULT] Compared with the control group, inflammatory responses, osteoclastogenesis, and bone resorption were much more obvious in the experimental group.[CONCLUSION] Bone implant in air pouch is an economic, rapid, and effective animal model to mimic the pathological process of aseptic loosening.PART II INTERVENTION OF THE ANIMAL MODEL[OBJECTIVE] To observe whether erythromycin and alendronate were effective in treating aseptic loosening in a murine osteolysis model.[METHOD] Air pouches were established on the back of BALB/c mice by injecting sterile air subcutaneously. After the air pouches were matured, a section of calvaria from a syngeneic mouse donor was implanted in the air pouch and polyethylene debris was injected into the air pouches. Mice were divided into 4 groups. In the first group, air pouches were injected with saline. In the other three groups, polyethylene debris was used in all the pouches. Saline, erythromycin and alendronate were injected intraperitoneally separately. Pouch tissues were collected 14 days after polyethylene debris inoculation for molecular and histology analysis.[RESULT] Both erythromycin and alendronate inhibited the osteoclastogenesis and bone resorption but alendronate was not as effective as erythromycin in anti-inflammation.[CONCLUSION] Both erythromycin and alendronate represent promising choice for the treatment of aseptic loosening. |
