Polymorphism Of CYP1A1/GSTM1 And Level Of Plasma Zinc/Copper Related With Non-Small Cell Lung Cancer

Objective: To study the relationship among the genetic polymorphism of CYP1A1 and GSTM1, environment exposure and susceptibility to non- small cell lung cancer so as to explore the mechanism of lung cancer and the new basis of diagnosis, prevention and therapy for lung cancer. Methods: The case-control study was conducted among 77 non- small cell lung cancer cases and 77 healthy controls, data of environment exposure were collected by uniform questionnaire, the CYP1A1 and GSTM1 gene genetic polymorphisms were detected by the method of PCR-RFLP. Results: The NSCLC patients were more seen in worker and peasant groups,there was a statistical correlation in smoking and NSCLC(P<0.01), while the frequency of smoking more than 20 years is higher than that less than 20 years,there was a high frequency of dust and toxicant exposure in cases than in controls (P<0.01), and the same in deep-fry fats eating(P<0.01), by contrary, frequency of proper drinking and drinking sanitized water frequently was lower in cases(P<0.01). There was no statistical difference of GSTM1 null genotype frequency between case and control,the same as CYP1A1 (P>0.05), and the combinative effect between CYP1A1 and GSTM1 is not obvious,there was a low-grade synergistic interaction between toxicant and tobacco exposure with CYP1A1(I/V and V/V) genotype,and the same between toxicant esposure with GSTM1 null genotype. Conclusion: This study revealed there was some correlation beween NSCLC and occupation, smoking, dust, toxicant exposure and deep-fry fats eating significantly increase the risk of non- small cell lung cancer,drinking sanitized water frequently was associated with the decreased risk of lung cancer, there was no obvious effect in CYP1A1 or GSTM1 alone with lung cancer. maybe there was an elevated risk of lung cancer in CYP1A1(I/V and V/V) genotype with exposure to smoking and toxicant, and GSTM1 null genotype with exposure to toxicant. Objective To study the correlation of polymorphism of CYP1A1 MspI,GSTM1 and plasma zinc,copper level in independently and in combination with the risk of NSCLC. Methods The case-control study was conducted among 77 lung cancer cases and 77 healthy controls.The CYP1A1 and GSTM1 genetic polymorphisms were detected by PCR-RFLP,while the plasma zinc,copper was determined by ICP-AES. Results There was no significant difference in polymorphism frequencies of CYP1A1 MspI or GSTM1 between the case and control.The genotypes were no significantly associated with the risk of lung cancer;Average plasma level of zinc or among the lung cancer cases was significantly lower than that among controls(P<0.01),while the lever of copper is higher;Comparing with those who had higher plasma zinc level(≥117.0ng/dL) with CYP1A1 I/I,the odds ratios of those who had lower plasma zinc(<117.0ng/dL) with CYP1A1 I/V or V/V was 7.60(P<0.01) and OR of those with GSTM1 null is 8.55(P<0.01);Comparing with those who had lower plasma copper level(<120.0ng/dL) with CYP1A1 I/I ,the odds ratios of those who had higher plasma copper(≥120.0ng/dL) with CYP1A1 I/V or V/V was 2.31(P>0.05) and OR of those with GSTM1 null is 1.84(P>0.05). Conclusion Polymorphism of CYP1A1 and GSTM1 was not associated with the risk of NSCLC;there was significant inverse association between plasma zinc level and this risk,while the copper is positive association.We suggested that there was a combined effect of polymorphism of CYP1A1 MspI,GSTM1 and palsma zinc level on NSCLC.