Effect On Immune And Pathological Changes Of Total Flavonoids Of Litsea Coteana On Synovlum Of Adjuvant Arthritis Rats

PURPOSE Through morphology, immunology, molecular biology to research total flavone of Litsea Coteana (TFLC)'s effect on adjuvant arthritis(AA) rats' inflammation and the regulation of immune function, in order to explore the relevant mechanisms of therapeutic effect that TFLC has on the AA rats.METHOD This experiment used freund's complete adjuvant(FCA) by foot subcutaneo usly injection into rats to established AA models. Thorugh HE staining, examine the synovial tissue of knee joint and the morphologic changes of synovial cell by electron microscope,analyze TFLC's effect on AA rats about secretory function and morphological changes, through immunohistochemistry, Western Blot etc. to explore TFLC's effect on vascular endothelial growth factor (VEGF), intercellular adhesion molecule-1(ICAM-1) in the synovial expression of the vascular wall.RESULTS TFLC (66.5,133,266mg·kg-1) can significantly reduce lateral ankle edema, inflammatory cell infiltration and angiogenesis on AA rats;TFLC (133,266 mg·kg-1)can resume secretory functions of both A&B type synovial cells and morphological abnormalities of Golgi,s apparatus, mitochondria, rough endoplasmic reticulum, can significantly reduce inflammatory cell infiltration and angiogenesis on AA rats' synovial cells;TFLC (66.5,133,266mg·kg-1) reduced AA rats synovial tissue VEGF, ICAM-1 protein expression .CONCLUSION TFLC can resume AA rats' synovial cells form, develop the secretory functions of synovial cells and the morphological abnormalities of some impo rtant organelles, reduce VEGF and ICAM-1 protein expression of synovial tissue,which is an important mechanism for the treatment of AA.