The Pharmaceutical Research Of Liyanling Mixture

The chronic pharyngitis is a throat familiar and frequently-occurring disease with patients'condition waving, lingering and difficult to cure. the cardinal symptoms is throat pain or redness. It has a bad influence on work and life of amplitude patients. The Chinese medicine thought that because of chronic pharyngitis more lung-kidney yin deficiency , the virtual fire caused by inflammation. Liyan Ling mixture is pure Chinese medicine oral administration agents,it's a clinical application of multi-year agreements prescription of the second hospital attached to Jilin University. According to Chinese medicine theory, combined with modern research and clinical experience,based on the combination of Drugs,it has good clinical effect.there are Flos lonicerae, Radix Rehmanniae Exsiccata,Radix Sophorae tonkinensis .Frutus A rctii and Radix Glycyrrhizae etc. in the mixture,prescription drugs are all contained in the "Chinese pharmacopoeia". Has raises promotes saliva or body fluids cloudy, the disintoxicating advantage swallows the effect,for the treatment of acute and chronic laryngitis.The topics to study Liyan Ling mixture preparation of standards and quality control. Selecting mixture as the preparation form,of a formulations,the dissertation included preparation process,quality standards,stability research and others by deep research. The concrete research is as follows:A the preparation procedureStudying the water extraction technics of Liyan Ling mixture by testing the contents of chlorogenic acid and Matrine . Using the orthogonal testing method, take soak time,solvent volume,extraction times,extraction time as the inspection factor, has carried on four factor three level orthogonal tests. Obtains the optimum extraction process,for medical material 1:8 by adding water,soak 12 hours,extracting three times, each time from one hour. Studying the water extraction-alcohol deposition technics of drugs by testing the content of chlorogenic acid and Matrine.Using the orthogonal testing method for optimization of the ingredients of alcohol-extraction process,the relative density and liquid alcohol concentration for the study,obtained the best craft for the water decoction fluid concentration when approximately was 1.12 (60℃measures) to the relative density, joins 95% ethyl alcohol to cause the precipitation so that the alcohol content of 60%.B the quality standards reasearchThe Identification TLC and HPLC quantitative research were well studied in this topic of Liyan Ling mixture .Flos lonicerae,Radix Rehmanniae Exsiccata,Radix Sophorae tonkinensis ,Frutus A rctii and Radix Glycyrrhizae in this preparation were identified by TLC , the experimental results indicate that TLC spots were clear , characteristic value,the reproduction quality was good,the negative did not have the disturbance,the methods were suitable to controll the qualitative distinction of Liyan Ling Mixture .The content of chlorogenic acid and matrine were qualitative determined by HPLC. the experimental result indicated that liquid chromatography's linearity, the accuracy,the reproduction quality,the Canadian type returns-ratio was good,and has formulated its content limit.their content limits were both regulated: the content of chlorogenic acid( C42H62O16)in Flos lonicerae and matrine in Radix Sophorae tonkinensis, chlorogenic acid is no less than 0.34mg and matrine is no less than 0.28mg. C the stability reasearchIn the preparation stable aspect, used the normal temperature experiment and the acceleration test to the preparation character,the load difference,the microorganism limit,pH value,the relative density,qualitative identification and the content determination. the normal temperature experiment to inspect for 3 months,he acceleration test to inspect for 3 months. there was no significant change results,show that the storage of goods be good in this storage time.