Study On Purification Of Solanesol From Discarded Tobacco Extract

Solanesol, a polyisoprenoid alcohol, mainly exist in nightshade, especially in tobacco leaves. Solanesol has a good effect on anti-bioactivity,anti-bacterium and dephlogisticate, which is an important intermediate material of medicine for curing cardiovascular disease, anticancer and anti-ulcer. High purity solanesol is a source of isoprene units for the synthesis of Coenzyme Q₁₀ and Vitamin K₂ analogs.Separation and purification of solanesol from discarded tobacco extract were studied in this dissertation.The summary of tobacco's composition and exploitation and purification was developed with brightly perspective in this paper. The purpose of this paper is separation and purification of solanesol from discarded tobacco extract. Firstly, the method for analyzing and detecting the content of solanesol by HPLC was studied. The pro-purification process of extracted solanesol has been developed in this work. Mainly three steps are contained in this procedure, i.e saponification, extraction and recrystalization. The optimal condition of saponification is as following: KOH solution as the saponifing catalyst, methanol as the diluent agent, saponifing over night at 50°C. For further purification, the condition of column chromatography, for example, the election of adsorbent,definition of eluant were investigated in this dissertation. Many factors including absorbent quantities, flow rate, raw material quantity, which effect column separation, were also investigated. The result shows the optimal separation condition of column chromatography is: the weight of silica gel is 30 g , the content of solanesol is 1.0 g, the flow rate is 0.6 ml/min. On the base of this method, the content of solanesol is 95% above.A model based on general rate (GR) has been developed for simulating the chromatographic separation of solanesol in a macroporous resin column. Parameters of axial dispersion, interfacial film mass transfer and intraparticle diffusion are included in the model. The performance of the model was verified by comparing the predictions of the model to the experimental data, the results show that the model can be used to guide the optimazation of operation conditions for the chromatographic separation of solanesol in a macroporous resin column.