The Experimental Study Of Neuronal Apoptosis Behind Salvia Injection Of Rat Facial Nerve Injury

Background: With the increase of all kinds of trauma, facial nerve injury has also increased the opportunities.Facial nerve injury caused by a series of changes and restoration of a research and maxillofacial surgery one of the focal points.Early in damage, more sensitive neurons in the first pathological changes, resulting in a series of clinical symptoms, such as delays in treatment, the body becomes pathological changes irreversible degenerative changes.At present large number of studies show that after nerve injury, nerve regeneration is mainly due to adverse secondary damage increasing, and secondary damage is more than one factor to participate in the process in which apoptosis plays a very important role.The results show that, not only in the development of the nervous system in the nervous system injury and degenerative diseases can also be found in the process of apoptosis. Research has indicated that in the secondary damage to the central nervous system in the process, due to injury tissue ischemia, hypoxia secondary pathological changes, resulting in tissue oxygen free radicals generated increased, decreased ability to remove, resulting in oxygen free radical accumulation.This oxidative stress (oxidative stress) can induce apoptosis, is a hub of secondary injury after pathological damage one of the main mechanisms. Salvia is improving microcirculation, and anti-lipid peroxidation, and increased activity of superoxide dismutase, remove blood and nerve injury in the free radicals and prevent calcium overload caused by the series of pathophysiological damage and inhibit brain cells The role of apoptosis.Facial nerve after peripheral nerve injury, facial nerve nuclear greater degree of influence, whether the peripheral nerve severed, can be connected directly affect the facial nerve nucleus neuron degeneration and apoptosis, and so on, direct impact on the function, aesthetics, which to injury repairing a certain particularity. At the same time a new drug or in the study stage, or more expensive, not widely used.With the special nature of facial nerve injury, and Salvia can effectively protect nerve tissue, and more economical and reasonable price, can provide a large number of products. Therefore choose to Salvia drug for the treatment of facial nerve severed with or without the same period matched the rat animal model to observe the clinical treatment of facial nerve injury Salvia prospects.Given the observation cell apoptosis, special choice of TUNEL staining immunohistochemistry (ie detection of apoptosis staining), parallel conventional HE staining. 2 choice Chromotrope R-Brilliant Green staining to observe the peripheral nerve fibers after injury myelin repair status. Final results will be discussed Salvia injection in the treatment of facial nerve injury and the possible mechanism of clinical application.Objective: To observe the facial nerve injury after application of Salvia miltiorrhiza on apoptosis, and to explore Salvia injection in the treatment of facial nerve injury may mechanism.Methods: 0.3ml/kg Sumianxin trip intramuscular anesthesia, in the supine and then fixed the right side in the first stage operation, under aseptic conditions in the surgery under a microscope revealed the left facial nerve.Facial nerve simply cut off group: facial nerve stem milk ear hole issued after the branch of the distal with 2 / 0 thread facial nerve ligation in the ligation of the distal 5 mm long with neural stem; facial nerve axotomy anastomosis group: the left facial nerve Milk-stem issued ears cut off after the distal branch of neural stem, and then immediately used 12 / 0 suture without injury tension-free conditions in the downstream side of the outer membrane - to-end anastomosis. Right facial nerve as normal controls. RSM group 30 min before each of peritoneal injection Salvia slow injection of 300 mg / kg, physiological salt group with the means to contour saline; the end of operation, gentamicin injection washing wound; rats after Salvia Salvia respectively intraperitoneal injection of 50 mg / kg, 2 times / d, in the saline group contour after saline injection, lasted 10 d, do not use in the normal group. Rats were sacrificed at the time, the conventional preparation of specimens, fixed, embedded, sliced.Results: 1, animal tentacles is blowing over the rhythm of the restoration: simply cut off the facial nerve in the test group rats observation time of the rhythm is blowing over the tentacles were not resumed. Facial nerve severed after the anastomosis is blowing over the rhythmic rat whiskers gradually restored, RSM group faster recovery.2, facial nerve, histological observation of the week: simply cut off the group of free self-organizing neural difficulties, the naked eye see large scar tissue, under axons showed swelling, collapsed, irregular shape, Schwann cell hyperplasia, a few larger cells, nuclear, myelin fracture. RSM group of lesser status. After anastomosis group cut off the outer membrane of nerve repair, replace a portion of scar tissue membrane inward growth, we can see that some of demyelinating reaction, and more infiltration of inflammatory cells, The situation of application RSM group is more minor than saline group .3, TUNEL Immunohistochemical detection of apoptosis + Chromotrope of R-2-stained light green mild observation: In the control group of rats after nerve injury in a facial nucleus d can be seen scattered in the TUNEL-positive cells, damage 3 d apoptosis index of more high, 7 d reached the peak, and then gradually decline Salvia rat facial nerve nuclear apoptosis index lower than the normal saline group, the peak has occurred in 7 d after injury, and then gradually decreased, see Figure 3,4. RSM group with normal saline group two different time points of difference between significant (P <0.05, P <0.01). Visible in the same period degeneration of neurons surrounding astrocytes see active cell proliferation, cell body hypertrophy, abundant cytoplasm, processes more extended, at home, in-stained TUNEL staining on the R-2 Chromotrope Brilliant Green, see cell coloring deepened. RSM group seen in the use of more saline group active response, even in a partial injury reactive gliosis.Conclusion: 1, Salvia injection can promote peripheral nerve fibers after injury repair in the central nervous system can be reduced neuronal degeneration and ease the process of apoptosis.2, Salvia injection can be effective inhibition of neuronal apoptosis.3, Salvia injection can to a certain extent, activated astrocytes, which many nerve tissue injury protection, which will help neuronal survival and regeneration.4, injury early on in the application of Salvia injection, clear long-term effect.