The Influence On Making-Blood Microenvironment About Marrows Transplanting Mice By Shengxue Soup

The marrow transplantation is one of the most effective measures to cure the blood diseases, the function obstacles on marrows making - blood, which are caused by the malignant substantive chemotherapy, and many kinds of blood-deficiency anomies. The course of the making-blood rebuilding after the marrow transplantation is one of many periods and factors. During the whole course, the renovation to making - blood microenvironment of the marrows influences the renewal, multiplication and differentiation by itself of the making - blood stem cells, as well as has the significance to the making-blood function of the organic rebuilding.Based on the documentary measures, this research sets up the marrow transplantation model by resorting to BALB/C mice and select Shengxue soup, which has the exact clinical curative effect, as curative recipe. And from the research, we can learn the marrow histology, the death of the marrow cells and the cells'cycle, the expressive level of the attached molecule ICAM-1 and the stem cell factor SCF within the marrow cells as well. What's more, the research analyzes the influence on making - blood microenvironment about marrows transplanting mice by Shengxue soup, probes into the likely applied mechanism of the traditional Chinese medicine prescription in the field of making-blood stem cell transplantation and affords the theoretical basis to develop further clinical applied prospect.The results of this research as follows:1.Shengxue soup can promote the marrow tissue's multiplication and differentiation of BMT mice, the capacity increase of the marrow cell making-blood tissues and the mature red blood cells, the capacity decrease of the adipose tissues, so that the marrow making-blood microenvironment develops effectively.2.Shengxue soup promotes BMT mice's marrow cells into the cell multiplication cycle and inhibits the death of the marrow cells.3.Shengxue soup can regulate BMT mice's marrow cells ICAM-1 and SCF,and develop their expressive level, which restores the damaged marrow microenvironment and accelerates the making-blood rebuilding. 4.The use of Shengxue soup and G-CSF has certain synergetic influence on promoting the multiplication and differentiation of BMT mice's marrow cells, inhibiting the death of the marrow cells and regulating the expression of SCF.