Study On Expression Regularity Of Muc-1,Muc-2 MRNA In Rat Intestinal Mucosa Cell Of IBD

Inflammatory bowel disease (IBD) is an agnogeically inflammatory disease of rectum and colon, including crohn disease (CD) and ulcerative colitis (UC). It also is an autoimmune disease of alimentary system. The lesion mainly occurs stratum mucosum and stratum submucosum. Diarrhea stomachace and blennoid bloody purulent sstool are the clinical manifestations. patients'conditions are different but most of those attack recurrently on the course of their chronic disease.The etiopathogenisis and nosogenesis are unclear now .Most researchers think they are related with infection, immunologic abnormality, heredity and so on .Varied predisposing factors provoke intestinal mucosa of the susceptibles and radical immunoinflammatory reaction occur. Recently the intimate relationship between functional abnormality of organism immunity and nosogenesis of IBD.Mucin (MUC) is a glycosylated protein with high molecular weight excreted by the epithelial cells of gastrointestinal ducts and ovary.The protective effect of mucin in intestinalinnunity are thinked more and more highly .Once the immunal function of these epithelial cell is disturbed, the quality and quantity of Mucin are changed.So far,there are fifteen mucins found .The study of Muc1 and MUC2 was clearer. We set up a trinitronenzene sulfonic acid (TNBS)-induced colitis model in rat and to execute rat on 3,7,14,30,60 day after TNBS/ethanol enema and to take colon tissue.appling Real-time Quantitative PCR to detect expression change of MUC1,MUC2 mRNA in etiopathogenisis of IBD and supply the experimental evidence for the researches on nosogenesis and clinic treatment of IBD.According to the Rat MUC1,MUC2 gene sequence designed prime and to quantitative research MUC1,MUC2 mRNA in colon tissue. The result showed MUC2 mRNA was significantly decreased (p<0.01), MUC1 mRNA was upgraded. We conclude MUC2 gene was inhibited in IBD.The induction of MUC2 participates in the occurrence and development of IBD.It may play an important role in the pathogegesis of Inflammatory bowel disease.Increasing of MUC1 mRNA expression may aggravate pathogenetic condition.