| .The prostate gland cancer is the masculine uropoiesis reproduction system common malignant tumor, In the European and American countries male population main causes of death. Our country prostate gland cancer disease incidence far is lower than the western one, but in recent years, with the medical health care facility's popularization and the improvement, the diagnosis technology's enhancement as well as the nutrition structure's change, it assumed the remarkable growth trend.The cytodynamics research confirmed, that prostate gland cancer arises results from the cell multiplication and weakly perishes the cell number increase and unbance. The cancer prevents and controls the cytology foundation is controlled by the tumor cell the infinite multiplication differentiation. The multiplication differentiation is regulated by the many kinds of factors, p21 cancergene is this kind of main multiplication differentiation regulation factor. Weakly perishes the mechanism core ingredient is kind ofprotein hydroltyic enzyme, presently the general designation is caspase. Caspase-3 and Caspase-9 is in the caspase family the most important member. The effective induction prostate gland cancer cell weakly perished has been valued people's day by day, is regarded as the research and development prostate gland cancer chemotherapy medicine and the chemotherapy plan fresh target.DDP is auti DNA duplication synthesis platinum the chemotherapy medicine, it by anti- tumor characteristic becomes one of the clinical most widely-used medicines, but its the kidney poison, the stomach toxicity, urged to spit the nature and the long-term use produces and so resists its clinicalpractice drug.3,3-diindolylmethane ( DIM) may cause the cell cycle to stagnate to period the G1 but cannot enter the periods, the suppressing cell multiplication, inducing it weakly to perish.AIM: Which were the platinum (DDP) and 3, 3- diindolylmethane(DIM) jointly used in prostate gland cancer cell PC-3, to observe its effect, expecting to enhance its curative effect, reduces its poisonous side effect, forms safe, effective and is stable jointly with the medicament, used medicine model in the anti- prostate gland cancer clinical treatment.METHODS:1) uses the MTT to exam PC-3 cell multiplication suppression situation.2) the acridine orange staining method to examine weakly perishes the cell.3) flowing cell technique to exam cell cycle and weakly perishess peak.4) RT-PCR examining DIM and' DDP influence on the P21,caspase-3 and caspase-9 expression5) Immunity groups checking examination caspase-3 and caspase-9 activeness.RESULTS:1) MTT law demonstration various dosage of DIM and DDP have inhibited action to thePC-3 cell, assumes the obvious dosage dependence, 0.4 mg.L-1 DDP +60mu mol.L-lDIM jointly for the medicine growth suppression rate (70.9%)nearly achieved the growth suppression rate which 4mg.L-lDDP .processes (71.6%).2) Acridine orange staining method: the cell weakly perishes when pyknosis, sends out a stronger loose skinned orange to be decadent or the redfluore scence, the chromatin dissemination to hyalomitome, presents the granulated fluore scence. Alone and jointly appears the loose skinned orange yellow and the loose skinned orange red fluorescence for the medicine group's PC-3 cell weakly perishes the cell, also jointly weakly perishes with the medicine group the cell number obviously to be many to alone uses the medicine group3) The cell apoptosis rafter DIM and DDP affect PC-3 cell 72h, ahead of time appears in Globviously weakly perishes the peak, DIM obviously strengthened the function which the DDP induction cell weakly perishes, center the dosage comes in handy group cell weakly perishing rate (45.9%) nearly to achieve high dosage DDP group weakly perishing rate (47%).4) the resuls of electricity swims Expression level has the varying degree with medicine group P21,Caspase-3 and Caspase-9 the enhancement, the union group enhances affects strong Yu Danyong the group.5) the cellular immunity group observed result for the cell but chernature brownish yellow coloration, this experimental result show edobviously enhances with the medicine group masculine gender rate.CONCLUSION: DIM, DDP alone and the union use may cause the PC-3 cell cycle to stagnate to the Gl time but cannot enter the person S time, thus suppresses the PC-3 cell multiplication, its mechanism with surrenders to the state in the PC-3 cell the P21 expression level related; DIM,DDP may induce the PC-3 cell weakly to perish, the two union application may get up the synergism, its mechanism and a line grain of body suffers injury, activates Caspase-9, Caspase-3 concerns. DIM unites DDP to be possible to enable DDP to obtain the very good treatment result at a smaller dosage, thus can reduce along the platinum poisonous side effect and the tumor drug resistance, causes DDP to apply has the security and widespread. |
