Impact Of Kidney Schemia-Reperfusion Injury On Brain Of Rats

Objective:To investigate the effect and mechanism on brain during kidney ischemiareperfusion injury of rats.Methods:A model of kidney I/R injury was established by imitation.Forty healthy male SD rats were randomly divided into 5 groups as following (n=8):the control group(CTL,the group of sham operation),60min reperfusion following ischemia 30min IR(30′+60′) group,60min reperfusion following ischemia 60min IR(60′+60′) group,60min reperfusion following ischemia 90min(IR(90′+60′) group),60min reperfusion following ischemia 120minIR(120′+60′) group.In each group the rats were killed to obtain samples of kidney and brain at the specified time points.The contents of Cr,BUN,MDA,GSH-PX in blood,MDA,GSH-PX,SDH,ATPase in kidney and MDA,GSH-PX,SDH,ATPase in brain were observed in each group.Results:Kidney ischemia-reperfusion injury resulted in significant injury of kidney and brain.With the prolongation of ischemia,the function of Renal have obviously injury.The increase of BUN,Cr and kidney coefficient in blood have obviously statistic significance in IR(30′+60′) group(P＜0.05),there were many free radical in blood,kidney and brain, the rising of MDA has statistic significance,the descent of GSH-PX has statistic significance(P＜0.05).With the prolongation of ischemia,kidney and brain have disorder of energy metabolism.In IR(120′+60′) group,there have obviously decrease of Na⁺-K⁺ ATPase,Ca²⁺ATPase and SDH.There were change of injury in kidney and brain by pathological section.Conclusion:A model of kidney I/R injury was succeed.Kidney I/R injury can result in lipid peroxidation and disorder of energy metabolism,even change of structure in brain.The free radical were produced can result in lipid peroxidation of brain during the process of kidney I/R injury,at the same time,the decrease of enzymatic activity,these were main reasons.