Mechanism Study Of Effect On Lung During Ischemia-reperfusion Of Kidney

Objection To investigate the impact of kidney ischemia-reperfusion on pulmonary of rate. Methods A model of kidney I/R injury was established by imitation. Forty healthy SD rats were randomly divided into 5 groups as following(n=8):the control group (the group of sham operation), 60min reperfusion following ischemia 30min (I30′+R60′group), 60min reperfusion following ischemia 60min (I60′+R60′group), 60min reperfusion following ischemia 90min (I90′+R60′group), 60min reperfusion following ischemia 120min (I120′+R60′group). In each group the rats were killed to obtain samples of pulmo at the specified time points. The contents of Cr, BUN, TNF-α, NE, MDA in blood, MDA, NO in kidney and pulmo and TNF-α, NE, MDA in BALF were observed in each group. Results Renal ischemia-reperfusion resulted in significant pulmonary injury. With the extension of ischemia-reperfusion, comparing with BUN and Cr in blood increased significantly in every control groups (P<0.01). There were many free radical in blood, kidney and pulmonary, the rising of MDA and NO have statistic significance. The content of TNF-αin blood and BALF increased in four control groups, especially in the latter two groups (P<0.01). NE in blood and BALF have increased in every control groups (P<0.05). There were change of injury in kidney and pulmonary by pathological section. Conclusion Renal ischemia-reperfusion can induce injury to pulmonary in the early stage of reperfusion. The results suggested that SIRS involved in the forming of pulmonary tissue injury after renal ischemia-reperfusion underlying the oxygen free radicals damage.